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Simple and rapid methods for labelling of human IgG by four radionuclides

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Abstract

Labelling of DTPA bicyclic anhydride coupled antibodies were investigated by determining the effect of DTPA: antibodies, DTPA: Sn molar ratios, pH, dimer and polymer formation of antibodies coupled with DTPA, using three different radionuclides, [111In,90Y and99mTc]. Analyses were performed with by Whatman No. 1 paper strips. Under optimal conditions we have achieved specific activities of111In or90Y labelled antibodies of about 37 kBq/1 μg for IgG coupled with about 2 DTPA groups per molecule and protein concentration of 15 mg/ml.

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References

  1. D. J. HNATOWICH, W. W. LAYNE, R. L. CHILDS, D. LANTEIGNE, M. A. DAVIS, Science, 220 (1983) 613.

    PubMed  Google Scholar 

  2. E. S. VITETTA, K. A. KROLICK, S. MIYAMA, Science, 219 (1983) 644.

    PubMed  Google Scholar 

  3. A. H. GOBUTY, E. E. KIM, J. Nucl. Med., 26 (1985) 546.

    PubMed  Google Scholar 

  4. D. J. HNATOWICH, R. L. CHILDS, D. LANTEIGNE, A. NAJAFI, J. Immuno Methods, 65 (1983) 147.

    Article  Google Scholar 

  5. A. NOWOTUY, Basic Exercises in Immunochemistry, 1979, p. 1.

  6. D. J. HNATOWICH, W. W. LUYNE, R. L. CHILDS, Int. J. Appl. Radiation Isotopes, 33 (1982) 327.

    Article  Google Scholar 

  7. D. J. HNATOWICH, F. VIRZI, P. W. DOHERTY, J. Nucl. Med., 26 (1985) 503.

    PubMed  Google Scholar 

  8. W. C. ECKELMAN, S. M. KARESH, R. C. REBA, J. Pharm. Sci., 64 (1975) 704.

    PubMed  Google Scholar 

  9. R. L. CHILD, D. J. HNATOWICH, J. Nucl. Med., 26 (1985) 293.

    PubMed  Google Scholar 

  10. Y. AKANO, Y. MAGATA, T. FURUKAWA et al., Report, IAEA-CN45, 1968, p. 143.

  11. H. FRANK, E. DELAND, E. KIM, D. M. GOLDENBERG, Cancer Res., 40 (1980) 2997.

    PubMed  Google Scholar 

  12. K. HUDSON, H. HAY, Pract. Immun., 00 (1980) 00.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Radioisotope Production Department, Nuclear Research Centre, P.O. Box 765, Baghdad, (Iraq)

    Y. F. Shafiq, S. A. Al-Safi, M. A. M. Abdel-Azeez, A. K. Galhoom & H. H. Samawi

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  1. Y. F. Shafiq
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  2. S. A. Al-Safi
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  3. M. A. M. Abdel-Azeez
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  4. A. K. Galhoom
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  5. H. H. Samawi
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Shafiq, Y.F., Al-Safi, S.A., Abdel-Azeez, M.A.M. et al. Simple and rapid methods for labelling of human IgG by four radionuclides. Journal of Radioanalytical and Nuclear Chemistry, Articles 147, 243–255 (1991). https://doi.org/10.1007/BF02040372

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  • DOI: https://doi.org/10.1007/BF02040372

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