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Radiolabeling of anti-carcinoembryonic antigen (CEA) monoclonal antibody (MAb BW 431/26) for clinical applications

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Abstract

A method for labeling of anti-carcinoembryonic antigen (CEA) monoclonal antibody (MAb) BW 431/26 is described. For preparations of99mTc-anti-CEA complex, a solution (1 ml) of tetrasodium-1,1,3,3-propanetetetraphosphate dihydrate (2.7 mg), stannous chloride dihydrate (0.12 mg) and sodium chloride (0.2 mg) in 5 ml of 0.9% saline was added to a vial containing monoclonal antibody (2 mg) from mouse (MAb BW 431/26), D-glucitol (2 mg) and sodium sulfate (2 mg) to obtain a clear solution. A quantitative labeling yield of the MAb BW 431/26 was achieved by addition of 5 ml (40.5 mCi, 1.5 Gbq) technetium-99m-pertechnetate (99mTcO4) generator eluate at room temperature within 10 minutes. The radiochemical purity determined by ITLC (Gelman, SG) plates was >95%.

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Authors and Affiliations

  1. Atomic Energy Medical Centre, Mayo Hospital, Lahore, (Pakistan)

    Hafiz Ghulam Abbas

  2. Institute of Nuclear Medicine and Oncology (INMOL), Lahore, (Pakistan)

    Saeeda Asghar & Maqbool Ahmad Shahid

Authors
  1. Hafiz Ghulam Abbas
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  2. Saeeda Asghar
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  3. Maqbool Ahmad Shahid
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Abbas, H.G., Asghar, S. & Shahid, M.A. Radiolabeling of anti-carcinoembryonic antigen (CEA) monoclonal antibody (MAb BW 431/26) for clinical applications. Journal of Radioanalytical and Nuclear Chemistry, Articles 189, 45–50 (1995). https://doi.org/10.1007/BF02040179

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  • DOI: https://doi.org/10.1007/BF02040179

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