The determination of selenium in urine

  • C. K. Baskett
  • V. L. Spate
  • M. M. Mason
  • C. L. Reams
  • J. S. Morris
Nuclear Analytical Methods in Biology and Medicine

Abstract

The selenium excreted in urine can be measured to assess the dietary status of selenium, an essential trace element in human nutrition. The objectives of this work were: 1) to develop a procedure, capable of high sample throughout, by which the major interferences can be reduced such that selenium concentrations can be measured in urine by Neutron Activation Analysis (NAA) using77mSe (17.4 s; and 2) to apply the method to a human dietary selenium study in which several selenium monitors were compared. The method involves a pre-irradiation arsenic-coprecipitation separation of the selenium from urine in the presence of a high specific-activity75Se tracer. The processed urine samples are analyzed using NAA. The procedure was applied to 58 urine specimens longitudinally collected from 12 subjects consuming three different levels of selenium. A dose-response relationship was observed in urine as well as a high correlations with both serum and whole blood selenium concentrations.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    K. SCHWARZ, C. M. FOLTZ, J. Am. Chem. Soc., 79 (1957) 3292.CrossRefGoogle Scholar
  2. 2.
    Subcommittee on the 10th Edition of the RDA Formed by the Food and Nutrition Board of the National Research Council, RDA: The Dietary Recommended Daily Allowances, 10th ed., National Academy Press, Washington D.C., 1989, p. 217.Google Scholar
  3. 3.
    R. F. BURK, Ann. Rev. Nutr., 3 (1983) 53.CrossRefGoogle Scholar
  4. 4.
    O. A. LEVANDER, B. SUTHERLAND, V. C. MORRIS, J. C. KING, Selenium in Biology and Medicine, AVI Publishing Company, Westport, Connecticut, 1981, Chapter 22.Google Scholar
  5. 5.
    C. K. BASKETT, Nuclear Analysis Program Documentation, MURR, Columbia, MO, 1990, Section IV. A. 1.Google Scholar
  6. 6.
    V. L. SPATE, Nuclear Analysis Program Documentation, MURR, Columbia, MO, 1990, Section IV. A. 6.Google Scholar
  7. 7.
    V. L. SPATE, Nuclear Analysis Program Documentation, MURR, Columbia, MO, 1990, Section IV. A. 8.Google Scholar
  8. 8.
    C. K. BASKETT, Nuclear Analysis Program Documentation, MURR, Columbia, MO, 1990, Section IV. A. 2.Google Scholar
  9. 9.
    M. P. LONGNECKER, M. J. STAMPFER, J. S. MORRIS, V. L. SPATE, C. K. BASKETT, M. M. MASON, W. C. WILLETT, Am. J. Epidemiol., (in press).Google Scholar
  10. 10.
    J. S. MORRIS, D. M. McKOWN, H. D. ANDERSON, M. MAY, P. PRIMM, M. CORDTS, D. GEBHARDT, S. CROWSON, V. SPATE, Selenium in Biology and Medicine, AVI Publishing Company, Westport, Connecticut, 1981, Chapter 46.Google Scholar
  11. 11.
    K. ZINN, Nuclear Analysis Program Documentation, MURR, Columbia, MO, 1990, Section IV. C. 5.Google Scholar
  12. 12.
    D. M. McKOWN, J. S. MORRIS, J. Radioanal. Chem., 43 (1978) 411.Google Scholar
  13. 13.
    E. BROWNE, R. B. FIRESTONE, Table of Radioactive Isotopes, John Wiley & Sons, Inc. New York, 1986.Google Scholar

Copyright information

© Akadémiai Kiadó 1994

Authors and Affiliations

  • C. K. Baskett
    • 1
  • V. L. Spate
    • 1
  • M. M. Mason
    • 1
  • C. L. Reams
    • 1
  • J. S. Morris
    • 1
  1. 1.Research Reactor CenterMissouri UniversityColumbiaUSA

Personalised recommendations