Preparation and characterization of humic and fulvic acid working standards—practical experience
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Two fractions of both fulvic acids (FA) and humic acid (HA) were prepared by fractionation method of Pierce and Felbeck5 involving acid hydrolysis of soil rests. This step increases recovery of both FA and HA considerably what suggest us need for slight modification of IHSS method in some cases. The weight loss, change in organic carbon content and visible spectra are figures of merit discussed. After detailed characterization these humic substances (HS) will serve as the working standards for study of interactions between organomercurials and organic part of soil.
KeywordsPhysical Chemistry Hydrolysis Inorganic Chemistry Organic Carbon Fractionation
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- 1.M. M. KONONOVA, Soil Organic Matter, its Nature, its Role in Soil Formation and in Soil Fertility, 2nd Eng. ed., Pergamon Press, Oxford, 1966, p. 408.Google Scholar
- 2.M. SCHNITZER and S. U. KHAN, Humic Substances in the Enviroment, Marcel Dekker, New York, 1972, p. 9–27.Google Scholar
- 3.F. Y. STEVENSON, Humus Chemistry, Genesis, Composition, Reactions, John Wiley, New York, 1982, p. 26–54.Google Scholar
- 4.K. KUMADA, Chemistry of Soil Organic Matter, Elsevier, Amsterdam, 1987, p. 9–27.Google Scholar
- 5.R. M. PIERCE and G. T. FELBECK, A Comparison of Three Methods of Extracting Organic Matter from Soils and Marine Sediments, in Humic Substances, Nieuwerluis, Pudoc, Wageninen 1972.Google Scholar
- 6.H. Y. Page, and H. M. S. DUTOIT, J. Am. Chem. Soc., 30 (1930) 1599Google Scholar
- 7.S. KUWATSUKA, A. WATANABE, K. ITOH and S. ARAI, Soil Sci. Plant Nutr., 38 (1992) 23.Google Scholar
- 8.P. JAVORSKÝ et al., Chemické rozbory v zemědelských laboratořích I., České Budějovice, 1985. (in Czech)Google Scholar
- 9.N. SENESI, Metal-Humic Substances Complexes in the Enviroment, in D. C. ADRIANO (Ed.), Biogeochemistry of Trace Metals, Lewis Publ., Boca RATON, U.S.A. 1992, p. 429.Google Scholar
- 10.G. G. CHOUDHRY, Humic Substances Structural Aspects and Photophysical and Free Radical Chacteristics, in O. HUTZINGER (Ed.), The Handbook of Enviromental Chemistry, vol. 1., Part. C., Springer-Verlag, Berlin 1984, p. 3.Google Scholar
- 11.R. L. MALCOLM, Anal. Chim. Acta, 232 (1990) 19.Google Scholar