Abstract
Human prostate specific antigen (hPSA) RIA was developed with hPSA and anti-PSA prepared in our laboratory. Its standard curve was linear with a sensitivity of 0.5 μ g/L. Serum PSA levels of 130 normal males ranged from 0 to 3.5 μ g/L (1.15±0.93 μ g/L), which are consistent with the results of other conventional RIA. The recovery, intra- and inter-assay coefficients of variation conform to the demands of RIA, and the results of 41 samples obtained by both the PSA RIA and PSA RIA of DPC were well correlated (γ=0.990). PSA level of 23 patients with prostatic carcinoma was 10∼400 μ g/L.
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M. K. BRAWER, M. P. CHETNER, J. BEATIE, D. M. BUCHNER, R. L. VESSELLA, P. H. LANGE, J. Urol., 147 (1992) 841.
F. LABRIE, A. DUPONT, P. SUBURU, L. CUSAN, M. TREMBLAY, J. EMOND, J. Urol., 147 (1992) 846.
M. C. WANG, L. A. VALENZULEA, G. P. MURPHY, T. M. CHU, Investigative Urol., 17 (1979) 159.
M. K. SCHWARTZ, J. NISSELBAUM, N. STONE, D. SCHWARTZ, R. STANKIEVIC, Clin. Chem., 33 (1987) 928.
R. C. ROCK, D. W. CHAN, D. BRUZEK, C. WALDRON, J. OESTERLING, P. WALSH, Clin. Chem., 33 (1987) 2257.
R. J. LIEDTKE, J. D. BATZER, Clin. Chem., 30 (1984) 649.
W. J. CATALONA, JAMA 270 (1993) 265.
W. J. CATALONA, D. S. SMITH, T. L. RATLIFF, J. W. BASLER, JAMA 270 (1993) 948.
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Zhong, W., Chen, L. & Wang, R. Human prostate specific antigen (hPSA) purification and establishment of hPSA radioimmunoassay. Journal of Radioanalytical and Nuclear Chemistry, Articles 206, 227–232 (1996). https://doi.org/10.1007/BF02039647
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DOI: https://doi.org/10.1007/BF02039647