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Magnetic microparticle antibodies and their application to RIAs

  • Radiopharmaceuticals, Labelled Compounds and RIA
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Abstract

Three types of magnetic microparticle antibodies were developed: 1) magnetic second antibody I (MSA-I) where the antibody molecules were directly immobilized by physical adsorption on Fe3O4 microparticles (magnetic nucleus, MN) 10nm±34% in diameter, 2) magnetic second antibody II (MSA-II) where the antibody molecules were immobilized by chemical coupling on the MN coated with polyacrolein, and 3) magnetic, first antibody (MFA-T3) where the anti-T3 antibody molecules were specifically immobilized by immunoadsorption to the second antibody molecules of the MSA-II. The optimal conditions for their preparation were elaborated. Some physical, chemical and immunological characteristics of these magnetic microparticles were described. The application of the MSA-I, MSA-II and MFA-T3 to RIAs for evaluation of thyroid function, such as triiodothyronine (T3), reverse T3(rT3), free T3 (fT3), thyroxine (T4), free T4(fT4), thyroid-stimulating hormone (TSH), thyroglobulin (TG) and TG-antibody (TG-Ab), etc., was reported.

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Authors and Affiliations

  1. Institute of Radiation Medicine, 27 Taiping Road, 100850, Beijing, P.R. China

    Shen Rongsen, Wang Renzhi, Xing Ruiyun, Li Yingoi & Zhou Fengqi

  2. Northern Institute of Immunological Reagents, No. A-20 Panjiamiao Huangtugang, Fengtai District, 100076, Beijing, P.R. China

    Jiang Shaohua, Lin Zhihao & Xu Banglei

Authors
  1. Shen Rongsen
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  2. Wang Renzhi
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  3. Xing Ruiyun
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  4. Li Yingoi
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  5. Zhou Fengqi
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  6. Jiang Shaohua
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  7. Lin Zhihao
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  8. Xu Banglei
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Additional information

It is a final report of Research Contract No. 6485/ RB and part of Coordinated Research Programme on “Antibodies Immobilized on Magnetic Particles for Radioimmunoassay and Immunoradiometricassay of Hormones”, supported by International Atomic Energy Agency.

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Rongsen, S., Renzhi, W., Ruiyun, X. et al. Magnetic microparticle antibodies and their application to RIAs. Journal of Radioanalytical and Nuclear Chemistry, Articles 206, 205–218 (1996). https://doi.org/10.1007/BF02039645

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  • DOI: https://doi.org/10.1007/BF02039645

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