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Characterization of biological macromolecules by electrophoresis and neutron activation

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Abstract

As analytical techniques have become more sensitive, the role of trace elements associated with biological macromolecules has become the subject of many studies in the past twenty years. Many biologically significant macromolecules, such as nucleic acids and proteins, have trace elements essential to their function and structure. Instrumental neutron activation analysis (INAA) contributes useful information in such studies. A procedure combining polyacrylamide gel electrophoresis (PAGE) with INAA and autoradiography has been developed to study biological macromolecules and their associated trace elements. Results from the application of this method to several metalloproteins are presented.

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Authors and Affiliations

  1. Center for Analytical Chemistry, National Bureau of Standards, 20899, Gaithersburg, MD, (USA)

    S. F. Stone, D. Hancock & R. Zeisler

Authors
  1. S. F. Stone
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  2. D. Hancock
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  3. R. Zeisler
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Stone, S.F., Hancock, D. & Zeisler, R. Characterization of biological macromolecules by electrophoresis and neutron activation. Journal of Radioanalytical and Nuclear Chemistry, Articles 112, 95–108 (1987). https://doi.org/10.1007/BF02037280

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  • DOI: https://doi.org/10.1007/BF02037280

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