Abstract
Radioiodinated insulin and thyroid stimulating hormones were prepared using 1,3,4,6-tetrachloro-3α, 6α-diphenylglycouril (Iodogen). Conditions of iodination like concentration of iodogen, reaction time, etc., were optimized to get maximum yield. Stability studies of iodogen coated tubes were carried out over a period of time. The dependence of iodination yield on varying amounts of activity and protein concentration were investigated. Iodination yield over a range of pH was also studied. The radiolabelled hormones prepared by this method were used in radioimmunoassay and were compared with tracers prepared by the Chloramine-T method.
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Pillai, M.R.A., Gupte, J.H., Jyotsna, T. et al. Preparation of radioidinated insulin and thyroid stimulating hormone using 1,3,4,6-tetrachloro-3α, 6α-diphenylglycouril (Iodogen) for radioimmunoassay. Journal of Radioanalytical and Nuclear Chemistry, Articles 116, 193–202 (1987). https://doi.org/10.1007/BF02037222
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DOI: https://doi.org/10.1007/BF02037222