Summary
A defective phage Φ80.1 was obtained which carries anamber suppressor locus (su y , most likely identical tosu III ofBrenner andBeckwith, 1965) and part of the tryptophan operon fromE. coli K 12. Various homo- and heterogenotes with respect to this suppressor locus have been isolated. The action of theamber suppressor locus onamber sites in the decarboxylase gene of the pyruvate dehydrogenase complex and onT 4 amber mutants was tested. In all cases the suppressor in its active state (su y +; i. e., suppressingamber sites) was dominant over the suppressor in the inactive (su y −) state. The presence of anotheramber suppressor (su II ) in the host did not alter the expression ofsu y towardsT 4 amber mutants. Further evidence was obtained for the specificity of theamber suppression. While remaining unsuppressed in strains harboringsu II , twoamber sites of the O0-type in one of the pyruvate dehydrogenase genes are suppressed bysu y in heterogenotessu II +/su y +.
The kinetics of the expression of the suppressor locus after infection with the defective Φ80.1 carryingsu y + was tested and compared for the production of pyruvate dehydrogenase andT 4 amber mutants. Comparable rates of suppressed synthesis of the enzyme appeared to be reached much later than the formation ofT 4 amber mutants.
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Herrn ProfessorGeorg Melchers zum 60. Geburtstag gewidmet.
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Dennert, G., Hertel, R., Deppe, G. et al. Action of anamber suppressor gene carried by phageΦ80 . Zeitschrift für Vererbungslehre 97, 243–254 (1965). https://doi.org/10.1007/BF02035930
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DOI: https://doi.org/10.1007/BF02035930