Journal of Radioanalytical and Nuclear Chemistry

, Volume 97, Issue 2, pp 309–316 | Cite as

Radioimmunoassay of somatotropin using second antibody polyethylene glycol combination separation system

  • G. Samuel
  • M. R. A. Pillai
  • U. K. Singh
  • C. N. Desai
  • R. S. Mani
Article

Abstract

We describe a radioimmunoassay procedure for human somatotropin using second antibody — polyethylene glycol (PEG) combination for the separation of antibody bound and free antigen. The assay is done by a single incubation of 18 hours or with one hour pre-incubation and 3 hours post-incubation at room temperature (25 °C) and uses 0.1 mℓ of serum sample. The assay covers a range of 0–40 μg/ℓ and has a sensitivity of 0.6 μg/ℓ of somatotropin. The assay is validated by inter-assay and intra-assay variations, recovery and parallelism tests.

Keywords

Physical Chemistry Inorganic Chemistry Polyethylene Glycol Serum Sample 

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Copyright information

© Akadémiai Kiadó 1986

Authors and Affiliations

  • G. Samuel
    • 1
  • M. R. A. Pillai
    • 1
  • U. K. Singh
    • 1
  • C. N. Desai
    • 1
  • R. S. Mani
    • 1
  1. 1.Radiopharmaceuticals DivisionBhabha Atomic Research CentreBombay(India)

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