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Phosphine reduced IgG: A new method for99mTc labeling immunoglobulins

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Abstract

A new technetium labeling method for immunoglobulin reduced with tris(2-carboxy-ethyl)phosphine hydrochloride is presented. The Sandoglobulina IgG source was assayed for purity and optimum reagent's concentration and incubation times were determined. It was purified by column chromatography and labelled with Sn2+ reduced technetium in the presence of MDP. The kit is easy to prepare, labeling efficiency is >(97±1.9) % and stable for 6 hours. The immunoreactivity of the99mTc-IgG was verified by electrophoresis and Western blot tests. The IgG retained its structure after both the reducing and labeling processes and it was the only labeled species.

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Authors and Affiliations

  1. Radiofarmacia, Departamento de Medicina Nuclear, Instituto Nacional de Nutrición Salvador Zubirán, INNSZ, México, D. F., Mexico

    C. Arteaga de Murphy & L. Meléndez-Alafort

  2. Laboratorio de Química Orgánica, Facultad de Química, Universidad de la Habana, Habana, Cuba

    O. Martínez-Rivero

  3. Departamento de Fisiología de la Nutrición, Instituto Nacional de Nutrición Salvador Zubirán, INNSZ, México, D. F., Mexico

    E. Gómez

  4. Departamento del Reactor y Materiales Radiactivos, Instituto Nacional de Investigaciones Nucleares, ININ, México, D.F., Mexico

    G. Ferro-Flores

Authors
  1. C. Arteaga de Murphy
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  2. L. Meléndez-Alafort
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  3. O. Martínez-Rivero
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  4. E. Gómez
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  5. G. Ferro-Flores
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Arteaga de Murphy, C., Meléndez-Alafort, L., Martínez-Rivero, O. et al. Phosphine reduced IgG: A new method for99mTc labeling immunoglobulins. J Radioanal Nucl Chem 220, 41–45 (1997). https://doi.org/10.1007/BF02035345

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  • DOI: https://doi.org/10.1007/BF02035345

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