Advertisement

Preparation and isolation of a homogeneous125I-cortisol derivative

  • Sh. Menon
  • G. Samuel
  • S. A. Balakrishnan
Article

Abstract

Tracer specificity plays an important role in the radioimmunoassay (RIA) of steroid homones. In this paper, we describe the preparation and purification of125I-labeled cortisol derivative with a carboxymethyloxime-histamine bridge. The investigation on the method of purification showed that HPLC could be adopted for the routine preparation of a pure, homogeneous tracer. The retention time observed in HPLC for125I-histamine-CMO-cortisol conjugate could be used as an index for qualitative and quantitative assessments.

Keywords

Physical Chemistry Purification HPLC Steroid Inorganic Chemistry 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    C. T. Sawin, Annals Intem. Med., 68 (1968) 624.Google Scholar
  2. 2.
    J. Nerup, Acta Endocrinol., 72 (1974) 142.Google Scholar
  3. 3.
    J. Marilyn, K. Painter, G. D. Niswender, Clin. Chem., 22 (1976) 1850.PubMedGoogle Scholar
  4. 4.
    J. I. Thorell, S. M. Larson, Radioimmunoassay and Related Procedures, C. V. Mosby Co. St Louis, 1978, p. 129.Google Scholar
  5. 5.
    E. Rolleri, M. Zannino, S. Orlandini, R. Malvano, Clin. Chim. Acta, 66 (1976) 319.PubMedGoogle Scholar
  6. 6.
    R. M. Allen, M. R. Redshaw, Steroids, 32 (1978) 467.PubMedGoogle Scholar
  7. 7.
    P. W. Nars, W. M. Hunter, J. Endocrinol., 57 (1973) 47.PubMedGoogle Scholar
  8. 8.
    P. Y. Wong, A. V. Mee, F. F. Kho, Clin. Chem., 25 (1979) 914.PubMedGoogle Scholar
  9. 9.
    P. Raposinho, C. Oliveira, A. Rodrigues, I. Santos, L. Patricio, Proc. IAEA Symp., Developments in RIA and related procedures, Vienna, 1991, p. 105.Google Scholar
  10. 10.
    W. M. Hunter, F. C. Greenwood, Nature, 194 (1962) 495.PubMedGoogle Scholar
  11. 11.
    V. Bhupal, R. S. Mani, J. Radioanal. Nucl. Chem., 107 (1986) 377.Google Scholar
  12. 12.
    G. Samuel, M. R. A. Pillai, U. K. Singh, C. N. Desai, R. S. Mani, J. Radioanal. Nucl. Chem., 97 (1986) 309.Google Scholar

Copyright information

© Akadémiai Kiadó 1997

Authors and Affiliations

  • Sh. Menon
    • 1
  • G. Samuel
    • 1
  • S. A. Balakrishnan
    • 1
  1. 1.Isotope DivisionBhabha Atomic Research CentreBombayIndia

Personalised recommendations