Preparation and isolation of a homogeneous125I-cortisol derivative

  • Sh. Menon
  • G. Samuel
  • S. A. Balakrishnan


Tracer specificity plays an important role in the radioimmunoassay (RIA) of steroid homones. In this paper, we describe the preparation and purification of125I-labeled cortisol derivative with a carboxymethyloxime-histamine bridge. The investigation on the method of purification showed that HPLC could be adopted for the routine preparation of a pure, homogeneous tracer. The retention time observed in HPLC for125I-histamine-CMO-cortisol conjugate could be used as an index for qualitative and quantitative assessments.


Physical Chemistry Purification HPLC Steroid Inorganic Chemistry 
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Copyright information

© Akadémiai Kiadó 1997

Authors and Affiliations

  • Sh. Menon
    • 1
  • G. Samuel
    • 1
  • S. A. Balakrishnan
    • 1
  1. 1.Isotope DivisionBhabha Atomic Research CentreBombayIndia

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