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Review on quantitative PCR assay

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Abstract

The important existing quantitative PCR techniques, including the classification, description, evaluation and effect factors have been reviewed. Emphases are put on the types and evaluation of quantitative internal standard substances as well as the theoretical consideration of PCR quantitative process. The purpose is reasonably to establish our own detection protocol for quantification of HCMV in clinical samples.

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References

  1. R. K. Saiki, S. Scharf, F. Faioona, K. B. Mullis, G. T. Horn, H. A. Erlich, N. Arnheim, Science, 230 (1985) 1350.

    PubMed  Google Scholar 

  2. A. C. Syvanen, M. Bengtstrom, J. Tenhunen, H. Soderlund, Nucleic Acids Res., 16 (1988) 11327.

    PubMed  Google Scholar 

  3. A. Landgraf, B. Reckmann, A. Pingoud, Anal. Biochem., 193 (1991) 231.

    PubMed  Google Scholar 

  4. M. Shibata, M. Terashima, H. Kimura, K. Kuzushima, J. Yoshida, K. Horibe, T. Morishima, Hum. Pathol., 23 (1992) 911.

    PubMed  Google Scholar 

  5. A. M. Vlieger, A. M. J. C. Medenblik, R. P. M. V. Gijlswijk, H. J. Tanke, M. V. D. Ploeg, J. W. Gratama, A. K. Raap, Anal. Biochem., 205 (1992) 1.

    PubMed  Google Scholar 

  6. A. C. Syvanen, H. Soderlund, Methods Enzymol., 218 (1993) 474.

    PubMed  Google Scholar 

  7. Y. E. Khudyakov, L. Gauir, J. Singh, P. Patel, H. A. Pields, Nucleic Acids Res., 22 (1994) 1320.

    PubMed  Google Scholar 

  8. B. K. Rawal, J. C. Booth, S. Fernado, P. D. Butcher, R. L. Powles, J. Virol. Methods, 47 (1994) 189.

    PubMed  Google Scholar 

  9. S. Oka, K. Urayama, Y. Hirabayashi, K. Ohnishi, H. Goto, K. Mitamura, S. Kimura, K. Shimada, Biochem. Biophys. Res. Commun., 167 (1990) 1.

    PubMed  Google Scholar 

  10. T. Ozawa, M. Tanaka, S. Ikebe, K. Ohno, T. Kondo, Y. Mizuno, Biochem. Biophys. Res. Commun., 172 (1990) 483.

    PubMed  Google Scholar 

  11. N. S. Hamblet, F. J. Castora, Biochem. Biophys. Res. Commun., 207 (1995) 839.

    PubMed  Google Scholar 

  12. R. A. Frye, C. C. Benz, E. Liu, Oncogene, 4 (1989) 1153.

    Google Scholar 

  13. J. Chelly, D. Montarras, C. Pinset, Y. B. Netter, J. C. Kaplan, A. Kahn, Eur. J. Biochem., 187 (1990) 691.

    PubMed  Google Scholar 

  14. D. E. Kellogg, J. J. Sninsky, S. Kwok, Anal. Biochem., 189 (1990) 202.

    PubMed  Google Scholar 

  15. A. M. Wang, M. V. Doyle, D. F. Mark, Proc. Natl. Acad. Sci., USA, 86 (1989) 9717.

    Google Scholar 

  16. S. Diviacco, P. Norio, L. Zentilin, S. Menzo, M. Clementi, G. Biamonti, S. Riva, A. Falaschi, M. Giacca, Gene, 122 (1992) 313.

    PubMed  Google Scholar 

  17. S. Menzo, P. Bagnarelli, M. Giacca, A. Manzin, P. Uaraldo, M. Clementi, J. Clin. Microbiol., 30 (1992) 1752.

    PubMed  Google Scholar 

  18. P. D. Siebert, J. W. Larrick, Nature, 359 (1992) 557.

    PubMed  Google Scholar 

  19. P. D. Siebert, J. W. Larrick, Biotechniques, 14 (1993) 244.

    PubMed  Google Scholar 

  20. D. Zipeto, F. Baldanti, D. Zella, M. Furione, A. Cavicchini, G. Milanesi, G. Gerna, J. Virol. Methods, 44 (1993) 45.

    PubMed  Google Scholar 

  21. H. E. Chehadeh, G. Zerlauth, J. W. Mannhalter, Biotechniques, 18 (1995) 26, 28.

    PubMed  Google Scholar 

  22. R. Thiery, P. Boutin, C. Arnauld, A. Jestin, Biotechniques, 18 (1995) 212.

    PubMed  Google Scholar 

  23. G. Gilliland, S. Perrin, K. Blanchard, F. Bunn, Proc. Natl. Acad. Sci., USA, 87 (1990) 2725.

    Google Scholar 

  24. J. C. Fox, P. D. Griffiths, V. C. Emery, J. Gen. Virol., 73 (1992) 2405.

    PubMed  Google Scholar 

  25. J. C. Fox, M. Kidd, P. D. Griffiths, P. Sweny, V. C. Emery, J. Gen. Virol., 76 (1995) 309.

    PubMed  Google Scholar 

  26. K. Henco, M. Heibey, Nucleic Acids Res., 18 (1990) 6733.

    Google Scholar 

  27. J. C. Fox, V. C. Emery, Rev. Med. Virol., 2 (1992) 195.

    Google Scholar 

  28. N. C. P. Cross, Br. J. Haematol., 89 (1995) 693.

    PubMed  Google Scholar 

  29. U. Reischl, B. Kochanowski, Mol. Biotechnol., 3 (1995) 55.

    PubMed  Google Scholar 

  30. Shen Rongsen, Ma Liren, Huang Haiyan, Liu Yaoqing, Xing Ruiyun, Yang Hong, Bull. Acad. Milit. Med. Sci., 19 (1995) 204 (Chinese).

    Google Scholar 

  31. R. J. Wiesner, B. Beinbrech, J. C. Ruegg, Nature, 366 (1993) 416.

    PubMed  Google Scholar 

  32. M. Becker-Andre, K. Hahlbrock, Nucleic Acids Res., 17 (1989) 9437.

    PubMed  Google Scholar 

  33. L. Raeymaekers, Anal. Biochem., 214 (1993) 582.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Radiation Medicine, 27 Taiping Road, 100850, Beijing, P. R. China

    Shen Rongsen & Ma Liren

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  1. Shen Rongsen
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  2. Ma Liren
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Shen, R., Liren, M. Review on quantitative PCR assay. J Radioanal Nucl Chem 221, 137–142 (1997). https://doi.org/10.1007/BF02035255

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  • DOI: https://doi.org/10.1007/BF02035255

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