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Preparation of radiolabeled clonidine for use in radioimmunoassay

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Abstract

The preparation of stable and immunoreactive radioiodinated clonidine for the development of a radioimmunoassay of clonidine is described. Tyrosylmethyl ester (TME) conjugate of carboxylic acid derivative of clonidine was radioiodinated using chloramine T as the oxidizing agent. The radioiodinated clonidine was purified by thin layer chromatography, gel filtration and solvent extraction. Radiochemical purity, specific activity and immunoreactivity were determined. The stability of the labeled compound during storage was tested in various solvents at different temperatures.

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Authors and Affiliations

  1. B. V. Patel PERD Centre, Thaltej, 380 054, Ahmedabad, India

    H. L. Bhalla

  2. Khandelwal Lab. Ltd., 400 033, Bombay, India

    R. Mathew

  3. Isotope Division, Bhabha Atomic Research Centre, 400 085, Bombay, India

    Grace Samuel

  4. Radiopharmaceuticals Operations, BRIT, 400 705, New Bombay, India

    N. Sivaprasad

Authors
  1. H. L. Bhalla
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  2. R. Mathew
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  3. Grace Samuel
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  4. N. Sivaprasad
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Bhalla, H.L., Mathew, R., Samuel, G. et al. Preparation of radiolabeled clonidine for use in radioimmunoassay. J Radioanal Nucl Chem 221, 93–95 (1997). https://doi.org/10.1007/BF02035248

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  • DOI: https://doi.org/10.1007/BF02035248

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