Skip to main content
SpringerLink
Log in

Butanol extracts from myelin fragments. III. SDS-urea gel electrophoretic studies

  • Pharmacologica
  • Published:
Experientia Aims and scope Submit manuscript

Summary

The butanol extracts from myelin fragments showing 5-HT binding capacity were studied by SDS-urea gel electrophoresis. 5 main bands were observed and their molecular weights were determined by the method of Ferguson relationship, and revealed that these extracts contained proteolipid protein, DM-20 and basic proteins. Furthermore, the reconstituted fraction with crude basic proteins and lipids showed the saturable binding capacity for C14·5-HT.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. S. Fiszer and E. De Robertis, J. Neurochem.16, 1201 (1969).

    Article  CAS  PubMed  Google Scholar 

  2. S.F.R. Godwin and J.M. Sneddon, J. Neurochem.25, 283 (1975).

    Article  CAS  PubMed  Google Scholar 

  3. R. Ishitani, A. Miyakawa, R. Saito and T. Iwamoto, Experientia33, 932 (1977).

    Article  CAS  PubMed  Google Scholar 

  4. J.M. Pasquini and E.F. Soto, Life Sci.11, 433 (1972).

    Article  CAS  Google Scholar 

  5. J. Folch-Pi and M. Lees, J. biol. Chem.191, 807 (1951).

    Article  CAS  Google Scholar 

  6. R. Ishitani, A. Miyakawa and T. Iwamoto, Jap. J. Pharmac.; in press (1978).

  7. V.P. Whittaker, I.A. Michaelson and R.J.A. Kirkland, Biochem. J.90, 293 (1964).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. D.S. Chan and M.B. Lees, Biochemistry13, 2704 (1974).

    Article  CAS  PubMed  Google Scholar 

  9. R.E. Martenson, G.E. Deibler and M.W. Kies, J. biol. Chem.244, 4268 (1969).

    Article  CAS  PubMed  Google Scholar 

  10. L.C. Mokrasch, Life Sci.6, 1905 (1967).

    Article  CAS  PubMed  Google Scholar 

  11. G.A. Banker and C.W. Cotman, J. biol. Chem.247, 5856 (1972).

    Article  CAS  PubMed  Google Scholar 

  12. A. Chrambach and D. Rodbard, Science172, 440 (1971).

    Article  CAS  PubMed  Google Scholar 

  13. E. Barbarese, P.E. Braun and J.H. Carson, Proc. natl Acad. Sci. USA74, 3360 (1977).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. H.C. Agrawal, R.M. Burton, M.A. Fishman, R.F. Mitchell and A.L. Prensky, J. Neurochem.19, 2083 (1972).

    Article  CAS  PubMed  Google Scholar 

  15. R. Ishitani, Y. Tobari and A. Miyakawa, in preparation.

  16. P.R. Carnegie, Nature229, 25 (1971).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacology, Faculty of Pharmaceutical Sciences, Josai University, 350-02, Sakado, Saitama, Japan

    R. Ishitani & A. Miyakawa

Authors
  1. R. Ishitani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Miyakawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Acknowledgment. We thank Prof. T. Iwamoto for his encouragement during the course of this study.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ishitani, R., Miyakawa, A. Butanol extracts from myelin fragments. III. SDS-urea gel electrophoretic studies. Experientia 34, 1605–1606 (1978). https://doi.org/10.1007/BF02034702

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02034702

Keywords

Search

Navigation