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Use of31P NMR to measure pH of membrane-enclosed solutions

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Summary

Phosphorus nuclear magnetic resonance was employed to measure the internal pH of unsonicated multilamellar liposomes. A steady transmembrane pH gradient could be obtained in the presence of sulfate and citrate anions. The effects of some uncouplers and ionophores on this controlled pH gradient were studied.

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References

  1. B. Chance and L. Mela, J. biol. Chem.241, 4588 (1966).

    Article  CAS  PubMed  Google Scholar 

  2. S. Addanki, F.D. Cahill and J.F. Sotos, J. biol. Chem.243, 2337 (1968).

    Article  CAS  PubMed  Google Scholar 

  3. R.G. Johnson and A. Scarpa, J. biol. Chem.251, 2189 (1976).

    Article  CAS  PubMed  Google Scholar 

  4. R.P. Casey, D. Njus, G.K. Radda and P.A. Sehr, Biochemistry16, 972 (1977)

    Article  CAS  PubMed  Google Scholar 

  5. D.W. Deamer, R.C. Prince and A.R. Crofts, Biochim. biophys. Acta274, 323 (1972).

    Article  CAS  PubMed  Google Scholar 

  6. G. Navon, S. Ogawa, R.G. Shulman and T. Yamane, Proc. natl Acad. Sci. USA74, 888 (1977).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. J.A. Cramer and J.H. Prestegard, Biochem. biophys. Res. Commun.75, 295 (1977).

    Article  CAS  PubMed  Google Scholar 

  8. A.C. McLaughlin, P.R. Cullis, J.A. Berden and R.E. Richards, J. magn. Res.20, 146 (1975).

    CAS  Google Scholar 

  9. M.J. Dawson, D.G. Gadian and D.R. Wilkie, J. Physiol.267, 703 (1977).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. H. Hauser, D. Oldani and M.C. Phillips, Biochemistry12, 4507 (1973).

    Article  CAS  PubMed  Google Scholar 

  11. R.N. Robertson and T.E. Thompson, FEBS Lett.76, 16 (1977).

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Biochemistry, Institute of Molecular Biology, State University of Utrecht, University Centre ‘De Uithof’, Padualaan 8, Transitorium 3, Utrecht, The Netherlands

    P. Palatini, B. De Kruijff & J. De Gier

  2. Istituto di Farmacologia dell'Università di Padova, Largo E. Meneghetti 2, I-35100, Padova, Italy

    P. Palatini

Authors
  1. P. Palatini
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  2. B. De Kruijff
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  3. J. De Gier
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Additional information

Acknowledgments. We wish to thank Dr P. Cullis for his generous gift of egg lecithin and critical evaluation of the manuscript. P. Palatini was a recipient of a short-term EMBO (European Molecular Biology Organisation) fellowship.

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Palatini, P., De Kruijff, B. & De Gier, J. Use of31P NMR to measure pH of membrane-enclosed solutions. Experientia 34, 1580–1582 (1978). https://doi.org/10.1007/BF02034686

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  • DOI: https://doi.org/10.1007/BF02034686

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