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Anti-myosin stains chromaffin cells

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Summary

The presence in fixed chromaffin cells of antigenic sites for a myosin antibody was demonstrated using immunofluorescence techniques. Tests on viable cells showed that at least some of the antigenic sites seem to be localized on or close to the cell surface and explained the cell agglutination that occurred with the addition of the myosin antibody to cells isolated by a method described in this paper.

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References

  1. T.D. Pollard and R.R. Weihing, CRC Crit. Rev. Biochem.,2, 1 (1974).

    Article  CAS  PubMed  Google Scholar 

  2. J.M. Trifaró, Neuroscience3 1 (1978).

    Article  Google Scholar 

  3. J.M. Trifaró, and C. Ulpian, Neuroscience1, 483 (1976).

    Article  PubMed  Google Scholar 

  4. K. Burridge and D. Bray, J. molec. Biol.99, 1 (1975).

    Article  CAS  PubMed  Google Scholar 

  5. J.M. Trifaró, A.C. Duerr and J.E.B. Pinto, Molec. Pharmac.12, 536 (1976).

    Google Scholar 

  6. R.C. Roberts, D.G. Makey and U.S. Seal, J. biol. Chem.241, 4907 (1966).

    Article  CAS  PubMed  Google Scholar 

  7. J.M. Trifaró and C. Ulpian, FEBS Lett.57, 198 (1975).

    Article  PubMed  Google Scholar 

  8. M. Goldman, in: Fluorescent Antibody Methods, New York, Academic Press, 1968.

    Google Scholar 

  9. J.M. Trifaró, A.M. Poisner and W.W. Douglas, Biochem. Pharmac.16, 2095 (1967).

    Article  Google Scholar 

  10. K. Olden, M. Willinghan and I. Pastan, Cell8, 383 (1976).

    Article  CAS  PubMed  Google Scholar 

  11. M. Willinghan, R.E. Ostlund and I. Pastan, Proc. natl Acad. Sci., USA71, 4144 (1974).

    Article  Google Scholar 

  12. A.E. Bolton and W.M. Hunter, Biochem. J.133, 529 (1973).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. J.E. Hesketh, D. Aunis, M. Pescheloque and P. Mandel, FEBS Lett.80, 324 (1977).

    Article  CAS  PubMed  Google Scholar 

  14. U. Gröschel-Stewart, J.H. Chamley, J.D. McConnell and G. Burnstock, Histochemistry47, 285 (1976).

    Article  PubMed  Google Scholar 

  15. J.W. Sanger, Proc. natl. Acad. Sci. USA72, 1913 (1975).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. T.D. Pollard, J. Cell Biol.67, 72 (1976).

    Google Scholar 

  17. E. Lazarides and K. Weber, Proc. natl. Acad. Sci., USA71, 4561 (1974).

    Article  Google Scholar 

  18. A.M. Poisner and J.M. Trifaró, Molec. Pharmac.3, 561 (1967).

    CAS  Google Scholar 

  19. S. Berl, S. Puszkin and W.J. Nicklas, Science179, 441 (1973).

    Article  CAS  PubMed  Google Scholar 

  20. J.H. Hartwig and T.P. Stossel, J. biol. Chem.250, 5696 (1975).

    Article  CAS  PubMed  Google Scholar 

  21. J.F. Ash and S.J. Singer, Proc. natl Acad. Sci., USA73, 4575 (1976).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. I. Costero, A. Chávez, L. Peralta, E. Monroy and F. Ramón, Texas Rep. Biol. Med.23, suppl. 1, 213 (1965).

    Google Scholar 

  23. W.W. Douglas, in: Handbook of Physiology, Endocrinology, vol. 6, p. 367. Ed. H. Blaschko, G. Sayers and A.D. Smith. American Physiological Society, 1975.

  24. J.M. Trifaró, A. Rev. Pharmac. Tox.17, 27 (1977).

    Google Scholar 

  25. J.A. Spudich and S. Watt, J. biol. Chem.246, 4866 (1971).

    Article  CAS  PubMed  Google Scholar 

  26. B.K. Hartman and S. Udenfriend, Molec. Pharmac.6, 85 (1970).

    CAS  Google Scholar 

  27. K. Weber and M. Osborn, J. biol. Chem.244, 4406 (1969).

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology and Therapeutics, McGill University, 3655 Drummond Street, H3G 1Y6, Montreal, Quebec, Canada

    J. M. Trifaró, C. Ulpian & H. Preiksaitis

Authors
  1. J. M. Trifaró
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  2. C. Ulpian
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  3. H. Preiksaitis
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Additional information

Acknowledgments. This work was supported by grants from the Medical Research Council of Canada. We thank Dr B. G. Livett for advice in the preparation of isolated cells, Drs J. Lowenthal and B. Collier for comments and Mrs J. Ritchie for technical assistance.

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Trifaró, J.M., Ulpian, C. & Preiksaitis, H. Anti-myosin stains chromaffin cells. Experientia 34, 1568–1571 (1978). https://doi.org/10.1007/BF02034678

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  • DOI: https://doi.org/10.1007/BF02034678

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