Summary
The serum extracts were purified by column-, thin layer- and high pressure liquid chromatography. Deuterated cholecalciferol and deuterated 25-hydroxycholecalciferol were used as internal standards. The quantitative analysis was performed using GC-mass fragmentography technique of TMS-ethers.
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The authors thank Dr. A. Fürst (F.Hoffmann-La Roche, Basel) for a sample of 3β-acetoxy-27-norcholest-5-en-25-one.
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Zagalak, B., Curtius, H.C., Foschi, R. et al. Mass fragmentographic determination of cholecalciferol and 25-hydroxycholecalciferol in human serum. Experientia 34, 1537–1539 (1978). https://doi.org/10.1007/BF02034657
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DOI: https://doi.org/10.1007/BF02034657