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Mass fragmentographic determination of cholecalciferol and 25-hydroxycholecalciferol in human serum

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Summary

The serum extracts were purified by column-, thin layer- and high pressure liquid chromatography. Deuterated cholecalciferol and deuterated 25-hydroxycholecalciferol were used as internal standards. The quantitative analysis was performed using GC-mass fragmentography technique of TMS-ethers.

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Zagalak, B., Curtius, H.C., Foschi, R. et al. Mass fragmentographic determination of cholecalciferol and 25-hydroxycholecalciferol in human serum. Experientia 34, 1537–1539 (1978). https://doi.org/10.1007/BF02034657

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