Abstract
We have described “Magnetic Microparticle Antibodies and Their Application to RIAs” in a recently published paper1. In this article operative parameters for the preparation of a magnetic second antibody (MSA-II) including results of purification of donky anti-rabbit (DxR) serum by an (NH4)2SO4 precipitation method, rates of recovery of products in preparation of magnetic nucleus (MN, Fe3O4 microparticle), in distillation of acrolein (AL) and in preparation of polyacrolein magnetic particle (PAMP), change in pH value of suspension irradiated before and after60Co γ-irradiation and volume of wet sediment in separation of magnetic particles by a magnetic separator, etc., as well as correlation of levels of quality control (QC) sera obtained with liquid-phase double antibody assay (LDA) and MSA-II assay during four years were supplementarily summarized. These operative parameters would be helpful to mastering the procedures for preparation and/or use of the magnetic particles. The better correlation of levels of QC sera for both the assays showed the reliability of the magnetic antibody.
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References
Shen Rongsen, Wang Renzhi, Xing Ruiyun, Li Yinzqi, Zhou Pencqi, Jiang Shaohua, Lin Zhihao, Xu Banglei, J. Radioanal. Nucl. Chem., 206 (1996) 205.
G. A. Herbert, P. L. Pelham, B. Pittman, App. Microbiol., 25 (1973) 26.
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This paper is a progress report of the Technical Contract No. 8955/R0/Deferred Programme Acrivities (DPA) 1994 from 1996-02-15 to 1997-02-14, supported by Intemational Atomic Energy Agency.
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Rongsen, S., Ruiyun, X., Fengqi, Z. et al. Parameters concerning the preparation and performance of a magnetic microparticle antibody. J Radioanal Nucl Chem 218, 131–133 (1997). https://doi.org/10.1007/BF02033990
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DOI: https://doi.org/10.1007/BF02033990