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A simplified method for the preparation of α-32P-labeled deoxyribonucleoside triphosphates

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Abstract

A method for preparing α-32P-labeled deoxyribonucleoside triphosphates ([α-32P]dNTP)* is described. By comparing the labeling procedure to that ofWalseth et al.1,2 the reaction of Nuclease P1 is saved in our experiments and only three steps are needed for a routine preparation, without decreasing the yield of [α-32P]dNTP which is over 92% based on32Pi. In this paper [α-32P]dATP is taken as an example to deseribe the labeling procedure, the suitability of our method has been discussed.

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References

  1. T. F. Walseth, R. A. Johnson, Biochim. Biophys. Acta., 562 (1979) 11.

    PubMed  CAS  Google Scholar 

  2. T. F. Walseth, P. S. T. Yuen, M. C. Moos, Methods Enzymol., 195 (1991) 29.

    PubMed  CAS  Google Scholar 

  3. P. Davanloo, A. H. Rosenberg, J. J. Dunn, F. W. Sudier, Proc. Natl. Acad. Sci., USA, 81 (1984) 2035.

    CAS  Google Scholar 

  4. A. Maxam, W. Gilbert, Methods Enzymol., 65 (1980) 499.

    Article  PubMed  CAS  Google Scholar 

  5. C. Y. Wan, T. A. Wilkins, Anal. Biochem., 223 (1994) 7.

    Article  PubMed  CAS  Google Scholar 

  6. V. Cameron, O. C. Uhlenbeck, Biochem., 16 (1977) 5120.

    Article  CAS  Google Scholar 

  7. P. F. Schendel, R. D. Wells, J. Biol. Chem., 248 (1973) 8319.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Atomic Energy, P.O. Box 275(39), 102413, Beijing, P.R. China

    F. Wu, Y. Wang & M. Yi

Authors
  1. F. Wu
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  2. Y. Wang
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  3. M. Yi
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Wu, F., Wang, Y. & Yi, M. A simplified method for the preparation of α-32P-labeled deoxyribonucleoside triphosphates. J Radioanal Nucl Chem 218, 93–95 (1997). https://doi.org/10.1007/BF02033980

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  • DOI: https://doi.org/10.1007/BF02033980

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