Abstract
Headspace gas chromatography was used to examine the volatile components of bovine vaginal secretions in three consecutive estrous cycles from each of four cows. Results indicated that there was an interesting peak with a retention of 2.7 min. It showed a successive rise and fall zero to three days before estrus. Gas chromatographic evidence and mass spectral data confirmed that this peak was acetaldehyde. Another unidentified compound with a retention time of 27 min was found to be unique to proestrus. It occurred two to three days prior to estrus, but was absent at any other time of the cycle.
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References
Albone, E.S. 1984. Mammalian Semiochemistry. John Wiley & Sons, Limited, Chichester. pp. 226–234.
Albone, E.S., Gosden, P.E., andWare, G.C. 1977. Bacteria as a source of chemical signals in mammals, pp. 35–43,in D. Müller-Schwarze and M.M. Mozell (eds.). Chemical Signals in Vertebrates. Plenum Press, New York.
Beauchamp, G.K., Doty, R.C., Moulton, D.G., andMugford, R.A. 1976. The pheromone concept in mammalian chemical communication, pp. 144–160,in R.L Doty (ed.). Mammalian Olfaction, Reproductive Processes and Behavior. Academic Press, New York.
Cole, H.H. 1930. A study of the mucus of the genital tract of the cow, with a special reference to the cycling changes.Am. J. Anat. 46:261–302.
Cormack, D.H. 1987. Histology, 9th ed. J.B. Lippincott, Philadelphia. pp. 641–644.
Esslemont, R.J., andBryant, M.J. 1974. Economic and husbandry aspects of the manifestation and detection of estrus in cattle.Agric. Dev. Advis. Serv. 13:19–25.
Gonzalez, S., De Ambrosini, V.M., De Nadra, M.M., De Holgado, A.P., andOliver, G. 1994. Acetaldehyde production by strains used as probiotics in fermented milk.J. Food Prot. 57:436–440.
Hammond, J. 1972. The Physiology of the Cow. University Press, Cambridge. pp. 50–57.
Hart, G.H., Mead, S.W., andRegan, W.M. 1946. Stimulating the sex drive of bovine males in artificial insemination.Endocrinology 39:221–223.
Hiremath, L.S., Kessler, P.M., Sasaki, G.C., andKolattukudy, P.E. 1992. Estrogen induction of alcohol dehydrogenase in the uropygial gland of mallard ducks.Eur. J. Biochem. 203:449–457.
Hradecky, P., Sis, R.F., andKlemm, W.R. 1983. Distribution of flehmen reactions of the bull throughout the bovine estrous cycle.Theriogenology 20:197–204.
Jacobs, V.L., Sis, R.F., Chenoweth, P.J., Klemm, W.R., Sherry, C.J., andCoppock, E.E. 1980. Tongue manipulation of the palate assists estrous detection in the bovine.Theriogenology 13:353–355.
Jeavons, C.M., andZeiner, A.R. 1984. Effects of elevated female sex steroids on ethanol and acetaldehyde metabolism in humans.Alcoholism 8:352–358.
Jones, R.L. 1981. Chemistry of semiochemicals involved in parasitoid-host and predator-prey relationships, pp. 239–250,in D.A. Nordlund, R.L. Jones, and W.J. Lewis, (eds.). Semiochemicals: Their Role in Pest Control. John Wiley & Sons, New York.
Kiddy, C.A., andMitchell, D.S. 1981. Estrus-related odors in cows: Time of occurrence.J. Dairy Sci. 64:267–271.
Kiddy, C.A., Mitchell, D.S., Bolt, D.J., andHawk, H.W. 1978. Detection of estrus-related odors in cows by trained dogs.Biol. Reprod. 19:380–395.
Kiddy, C.A., Mitchell, D.S., andHawk, H.W. 1984. Estrous-related odors in body fluids of dairy cows.J. Dairy Sci. 67:388–391.
Klemm, W.R., Hawkins, G.N., andDe Los Santos, E. 1987. Identification of compounds in bovine cervico-vaginal mucus extracts that evoke male sexual behavior.Chem. Senses 12:77–87.
Klemm, W.R., Rivard, G.F., andClement, B.A. 1994. Blood acetaldehyde fluctuates markedly during bovine estrous cycle.Anim. Reprod. Sci. 35:9–26.
Maarse, H., andKepner, R.E. 1970. Changes in composition of volatile terpenes in Douglas fir needles during maturation.J. Agric. Food Chem. 18:1095–1099.
Müller-Schwarze, D., Morehouse, L., Corradi, R., Zhao, C.H., andSilverstein, R.M. 1986. Odor images: Response of beaver to castoreum fractions, pp. 561–570,in D. Duvall, D. Müller-Schwarze and Silverstein, R.M. (eds.). Chemical Signals in Vertebrates, Vol. 4, Ecology, Evolution, and Comparative Biology. Plenum Press, New York.
Nishimura, K., Utsumi, K., andOkano, T. 1991. Separation of mounting-inducing pheromones of vaginal mucus from estrual heifer.J. Anim. Sci. 69:3343–3347.
O'Connell, R.J., Singer, A.G., Macrides, F., Pfaffmann, C., andAgosta, W.C. 1978. Responses of the male golden hamster to mixtures of odorants identified from vaginal discharge.Behav. Biol. 24:244–255.
Paleologou, A.M. 1977. Detecting estrus in cows by a method based on bovine sex pheromone.Vet. Rec. 100:319–320.
Prescicce, G.A., Brockett, C.C., Cheng, T., Foote, R.H., Rivard, G.F., andKlemm, W.R. 1993. Behavioral responses of bulls in artificial breeding to compounds presented for smelling, licking or with topical nasal application.Appl. Anim. Behav. Sci. 37:273–284.
Qulali, M., andCrab, D.W. 1992. Estradiol regulates class I alcohol dehydrogenase gene expression in renal medulla of male rats by posttranscriptional mechanism.Arch. Biochem. Biophys. 297:277–284.
Qulali, M., Ross, R.A., andCrab, D.W. 1991. Estradiol induces class I alcohol dehydrogenase activity and mRNA in kidney of rats.Arch. Biochem. Biophys. 288:406–413.
Rivard, G., andKlemm, W.R. 1992. Two body fluids containing estrous pheromone(s).Chem. Senses, 14:273–279.
Vedamuthu, E.R. 1982. Fermented milks, p. 199,in A.H. Rose (ed.). Fermented Foods in Economic Microbiology. Academic Press, New York.
Williamson, N.B., Morris, R.S., Blood, D.C., andCannon, C.M. 1972. A study of oestrus behavior and oestrus detection methods in a large commercial dairy herd.Vet. Rec. 91:50–58.
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MA, W., Clement, B.A. & Klemm, W.R. Cyclic changes in volatile constituents of bovine vaginal secretions. J Chem Ecol 21, 1895–1906 (1995). https://doi.org/10.1007/BF02033850
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DOI: https://doi.org/10.1007/BF02033850