The expression of parathyroid hormone-related protein in human breast cancer with skeletal metastases
The relationship between the expression of parathyroid hormone-related protein (PTHrP) by breast cancer and skeletal metastases, was investigated using a monoclonal antibody against human PTHrP (4133). The immunohistochemical localization of PTHrP was studied in sections of formalin-fixed, paraffin-embeded tissues from 28 breast cancers obtained surgically between 1980 and 1985. Of the 28 patients, 12 developed skeletal metastases, 8 developed lung metastases, and the other 8 were alive and disease-free at the time of this study. Sixteen of the 28 (57%) tumors showed positive immunoreactivity to 4133, the PTHrP positive ratio being 83% in the patients who developed skeletal metastases, 38% in those who developed lung metastases, and 38% in those without recurrence, respectively. Thus, a significantly higher proportion of the patients who developed skeletal metastases were positive for PTHrP than the other two groups (P < 0.05). Furthermore, the level of positive staining was strongly related to positivity for estrogen and progesterone receptors (P < 0.01). These results are consistent with the hypothesis that PTHrP might be necessary for metastases to erode bone and grow in skeletal sites, and its expression could be related to certain hormones.
Key Wordsparathyroid hormone-related protein breast cancer skeletal metastases
Unable to display preview. Download preview PDF.
- 2.Mundy GR, Martin TJ (1983) The hypercalcemia of malignancy: Pathogenesis and management. Metabolism 31:1247–1227Google Scholar
- 4.Manngin M, Webb AC, Dreyer BE, Posillico JT, Ikeda K, Weir EC, Stewart AF, Bander NH, Milstone L, Barton DE, Francke U, Broadus AE (1988) Identification of a cDNA encoding a parathyroid hormone-like peptide from a human tumor associated with humoral hypercalcemia of malignancy Proc Natl Acad Sci USA 85:597–601PubMedGoogle Scholar
- 5.Stewert AF, Wu T, Goumas D, Burtis WJ, Broadus AE (1987) N-adenylcyclase-stimum terminal amino acid sequence of two novel tumor-derived adenylate cyclase-stimulating proteins: Identification of parathyroid hormone-like and parathyroid hormone unlike domains. Biochem Biophys Res Commun 146:672–678PubMedGoogle Scholar
- 10.Thomus AN, Loken HF, Goldman L (1960) Hypercalcemia of metastatic breast cancer. Surg Forum 16:70–71.Google Scholar
- 14.Untch M, Harback N, Eiermann W (1988) Micrometastasis in bone marrow in patients with breast cancer. Br Med J 296:290Google Scholar
- 15.Elston CW, Blarney RW, Johnson J (1980) The relationship of estradiol receptor (ER) and histological tumor differentiation with prognosis in human primary breast carcinoma. In: Mouridsen HT, Palshof T (eds) Breast cancer; experimental and clinical aspects. Pergamon, Oxford, p 59Google Scholar
- 20.Pahuja DN, DeLuca HF (1981) Stimulation of intestinal calcium transport and bone calcium mobilization by prolactin in vitamin D-deficient rats. Science 214:1058–1039Google Scholar