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Use of hepatitis B core antigen produced inEscherichia coli to detect immunoglobulin M specific antibodies in an enzyme-linked immunosorbent assay

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Abstract

The antigenic activity of HBcAg produced inEschericha coli and HBcAg from human liver was compared in aμ-specific solid-phase antibody-capture assay for detection of anti-HBc-IgM. HBcAg from liver could be detected in dilutions up to 1∶3, HBcAg fromEscherichia coli in dilutions up to 1∶10, 000. Using HBcAg fromEschericha coli, sera from five patients with acute resolving hepatitis B and sera from four patients with actue hepatitis B who had developed chronic liver disease were tested for anti-HBc-IgM in ELISA. IgM fractions separated out of the same sera by immunoaffinity chromatography were tested for anti-HBc-IgM using a commercially available test. The results were in good agreement with those obtained by ELISA. Anti-HBc-IgM could be detected up to 900 days after onset of disease. Different groups of patients were tested for presence of anti-HBc-IgM in ELISA. Fifty-nine of 60 patients with acute hepatitis B were positive for anti-HBc-IgM at onset of illness. Ten of 16 patients with chronic aggressive hepatitis and seven of 23 HBsAg positive dialysis patients were also positive for anti-HBc-IgM, whereas only two of 12 patients with chronic persistent hepatitis and one of 15 HBsAg positive blood donors (“healthy” carriers of HBsAg) had detectable anti-HBc-IgM.

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Authors and Affiliations

  1. Departments of Medical Virology and Gastroenterology, University of Heidelberg, Im Neuenheimer Feld 324, 6900, Heidelberg, FRG

    G. Hasche, J. Stecher, K. Gmelin & H. W. Doerr

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  1. G. Hasche
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  2. J. Stecher
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  3. K. Gmelin
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  4. H. W. Doerr
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Hasche, G., Stecher, J., Gmelin, K. et al. Use of hepatitis B core antigen produced inEscherichia coli to detect immunoglobulin M specific antibodies in an enzyme-linked immunosorbent assay. Eur. J, Clin. Microbiol. 3, 30–34 (1984). https://doi.org/10.1007/BF02032811

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  • DOI: https://doi.org/10.1007/BF02032811

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