Lasers in Medical Science

, Volume 5, Issue 1, pp 17–20 | Cite as

Determinants of the fluorescence emission spectrum of atheromatous plaques treated with haematoporphyrin in vitro

  • David Kessel
  • Ramon Berguer


Studies were carried out on atheromatous plaques which had been incubated with the fluorescent dye haematoporphyrin. By varying the wavelength of excitation, it was possible to obtain fluorescence signals from different depths in plaque, since enhanced tissue penetration occurs when the wavelength of exciting light is increased. Moreover, the presence of ulcerated regions altered both excitation and emission spectra. These results suggest that corresponding measurements using laser/fibre-optic systems may be used to characterize plaques in vivo.

Key words

Fluorescence Plaques Haematoporphyrin Spectroscopy 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Clarke RH, Isner JM, Gauthier T et al. Spectroscopic characterization of cardiovascular tissue.Lasers Surg Med 1988,8:45–9PubMedGoogle Scholar
  2. 2.
    Hoyt CC, Richardds-Kortum RR, Costello B et al. Remote biomedical spectroscopic imaging of human artery wall.Lasers Surg Med 1988,8:1–9Google Scholar
  3. 3.
    Kittrell C, Willett RL, de Los Santos C et al. Diagnosis of fibrous arterial atherosclerosis using fluorescence.Appl Opt 1985,42:2280Google Scholar
  4. 4.
    Sartori M, Sauerbrey R, Kubodera S et al. Autofluorescence maps of atherosclerotic human arteries—a new technique in medical imaging.IEEE J Quant Elec 1987,QE23:1794CrossRefGoogle Scholar
  5. 5.
    Spokojny A, Serur J, Skillman J, Spears JR. Uptake of hematoporphyrin derivative by atheromatous plaques; studies in human in vitro and rabbit in vivo.J Am Coll Cardiol 1986,8:1387–92PubMedGoogle Scholar
  6. 6.
    Spears JR, Serur J, Shropshire D, Paulin S. Fluorescence of experimental atheromatous plaques with hematoporphyrin derivative.J Clin Invest 1983,71:395–9PubMedGoogle Scholar
  7. 7.
    Kessel D, Sykes E. Porphyrin accumulation by atheromatous plaques of the aorta.Photochem Photobiol 1984,40:59–61PubMedGoogle Scholar
  8. 8.
    Pollock ME, Eugene J, Hammer-Wilson M, Berns MW. Photosensitization of experimental atheromas by porphyrin.J Am Coll Cardiol 1987,9:629–46Google Scholar
  9. 9.
    Prevosti LG, Wynne JJ, Becker CG et al. Laser-induced fluorescence detection of atherosclerotic plaque with hematoporphyrin derivative used as an exogenous probe.J Vasc Surg 1988,7:500–6CrossRefPubMedGoogle Scholar
  10. 10.
    Morcos NC, Berns MW, Henry WL. Phycocyanin: laser activation, cytotoxic effects, and uptake in human atherosclerotic plaque.Lasers Surg Med 1988.8:10–17PubMedGoogle Scholar
  11. 11.
    Dougherty T. Photosensitizers: therapy and detection of malignant tumors.Photochem Photobiol 1987,45:879–90PubMedGoogle Scholar
  12. 12.
    Dougherty T. Photodynamic therapy.Clin Chest Med 1985,6:219–36PubMedGoogle Scholar
  13. 13.
    Kessel D, Berguer R. Determinants of porphyrin fluorescence emission spectra of atheromatous plaques.Atherosclerosis 1988,69:1–4CrossRefPubMedGoogle Scholar
  14. 14.
    Doiron D, Svaasand L, Profio AE. Light dosimetry in tissue: application to photoradiation therapy. In: Kessel D, Dougherty TJ (eds)Porphyrin Photosensitization. New York: Plenum Press 1983:63–76Google Scholar
  15. 15.
    Kessel D, Thompson P. Purification and analysis of hematoporphyrin and hematoporphyrin derivative by gel exclusion and reverse-phase chromatography.Photochem Photobiol 1987,46:1023–26PubMedGoogle Scholar

Copyright information

© Baillière Tindall 1990

Authors and Affiliations

  • David Kessel
    • 1
  • Ramon Berguer
    • 2
  1. 1.Department of PharmacologyWayne State University School of MedicineDetroitUSA
  2. 2.Department of SurgeryWayne State University School of MedicineDetroitUSA

Personalised recommendations