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Antirheumatic drugs ands macrophage function: Effects on tumour cell crowth in vitro

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Summary

The ability of the antirheumatic drugs D-penicillamine, chloroquine and levamisole to modify macrophage-mediated inhibition of tumour cell growth in vitro was investigated. Increasing numbers of rat peritoneal macrophages were cocultured with a fixed number of ascites hepatoma AH-13 rat tumour cells. Tumour cell growth was assessed as the uptake of3H-thymidine (3H-TdR) by AH-13 cells at the end of a 24h period of coculture with macrophages treated in vitro or in vivo with the various drugs. In vitro, preincubation of macrophages with D-penicillamine or chloroquine (50 – 250 μg/ml) increased tumour cell3H-TdR incorporation, compared to cultures with untreated macrophages. Macrophages from rats treated with D-penicillamine or chloroquine (50 mg/kg/day orally) for 4 days similarly increased tumour cell3H-TdR incorporation, compared to cultures with macrophages from untreated rats. These effects persisted for at least 3 to 4 weeks of treatment. Preincubation with levamisole (10 – 100 μg/ml) in vitro had no effect on macrophage-mediated inhibition of tumour cells, whereas increased tumour cell3H-TdR incorporation was observed in cultures with macrophages from rats treated with levamisole (5 mg/kg/day orally) in vivo. Macrophages from rats with experimentally induced chronic inflammation, i.e. adjuvant arthritis, were found to increase tumour cell3H-TdR incorporation, compared to macrophages from nonarthritic rats. This trend was further enhanced by treatment with D-penicillamine, chloroquine or levamisole.

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Authors and Affiliations

  1. Leo Pharmaceutical Products, Department of Pharmacology, DK- 2750, Ballerup, Denmark

    L. Binderup, E. Bramm & E. Arrigoni-Martelli

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  1. L. Binderup
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  2. E. Bramm
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  3. E. Arrigoni-Martelli
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Binderup, L., Bramm, E. & Arrigoni-Martelli, E. Antirheumatic drugs ands macrophage function: Effects on tumour cell crowth in vitro. Clin Rheumatol 1, 15–22 (1982). https://doi.org/10.1007/BF02032471

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