Clinical Rheumatology

, Volume 9, Issue 2, pp 182–185 | Cite as

Spontaneous activation of heat shock protein gene transcription in peripheral blood mononuclear cells of individuals with active systemic lupus erythematosus

  • Y. Deguchi
  • S. Kishimoto


In the present study, we show the spontaneous elevation of the transcription of heat shock protein (hsp 70) gene in peripheral blood mononuclear cells (PBMC) of individuals with active systemic lupus erythematosus (SLE) by nuclear run-on transcription assay. For quantification analysis of hsp 70 gene transcription, we first found more than ten times of the enhanced transcription of hsp 70 gene in PBMC of individuals with active SLE, as compared to normal healthy subjects or patients with bronchial asthma as controls. Our observation suggests that heat shock proteins may be produced during an active immune response in individuals with active systemic lupus erythematosus and is involved in lupus-related change of possible essential function in PBMC.

Key words

Heat Shock Protein Gene Transcription Systemic Lupus Erythematosus 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Jiminez, S.A. Cellular immune dysfunction and the pathogenesis of scleroderma. Semin Arthritis Rheum 1983, 13, 104–120.CrossRefPubMedGoogle Scholar
  2. 2.
    Hichey, E.D., Weber, L.A. Modulation of heat shock polypeptide synthesis in Hela cells during hyperthemia and recovery. Biochemistry 1982, 21, 1513–1521.CrossRefPubMedGoogle Scholar
  3. 3.
    Morange, M., Din, A., Bensude, O., Babinet, C. Altered expression of heat shock proteins in embryonal carcinoma and mouse early embryonic cells. Mol Cell Biol 1984, 4, 730–735.PubMedGoogle Scholar
  4. 4.
    Schlesinger, M.J., Aliperti, G., Kelly, P.M. The response of cells to heat shock. Trends Biochem Sci 1982, 7, 222–225.CrossRefGoogle Scholar
  5. 5.
    Currie, R.W., White, F.P. Trauma-induced protein in rat tissues. Science 1981, 214, 72–73.PubMedGoogle Scholar
  6. 6.
    Lindquist, S. The heat shock response. Ann Rev Biochem 1986, 55, 1151–1191.CrossRefPubMedGoogle Scholar
  7. 7.
    Lindquist, S., Craig, E.A. The heat shock response. Ann Rev Genet 1988, 22, 631–677.CrossRefPubMedGoogle Scholar
  8. 8.
    Tan, E.M., Cohen, A.S., Fries, J.F. et al. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1982, 25, 1271–1277.PubMedGoogle Scholar
  9. 9.
    Marzluff, W.F., Huang, R.C.C. Transcription and Translation; a Practical Approach. Oxford, I.R.L. Press, 1984, pp. 89–130.Google Scholar
  10. 10.
    Reed, J.C., Tsujimoto, Y., Alpers, J.D., Croce, C.M., Nowell, P.C. Regulation of bcl-2 protooncogene expression during normal human lymphocyte proliferation. Science 1987, 236, 1295–1299.PubMedGoogle Scholar
  11. 11.
    Schlesinger, M.J. Heat shock proteins: The search for functions. J Cell Biol 1986, 103, 321–325.CrossRefPubMedGoogle Scholar
  12. 12.
    Anathan, J., Goldberg, A.L., Voellmy, R. Abnormal proteins serve as eukaryotic stress signals and trigger the activation of heat shock genes. Science 1988, 232, 522–524.Google Scholar
  13. 13.
    Lindquist, S. Varying pattern of protein synthesis in Drosophila during heat shock. Dev Biol 1980, 77, 463–479.CrossRefPubMedGoogle Scholar
  14. 14.
    Pelham, H.R.B. A regulatory upstream promoter element in the Drosophila hsp 70 heat shock gene. Cell 1982, 30, 517–528.CrossRefPubMedGoogle Scholar
  15. 15.
    Parker, C.S., Topol, J. A drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene. Cell 1984, 37, 273–283.CrossRefPubMedGoogle Scholar
  16. 16.
    Kingston, R.E., Baldwin, A.S., Sharp, P.A. Regulation of heat shock protein 70 gene expression by c-myc. Nature 1984, 315, 280–282.CrossRefGoogle Scholar
  17. 17.
    Granelli-Piperno, A., Andrus, L., Steinman, R.M. Lymphokine and nonlymphokine mRNA levels in stimulated human T cells. J Exp Med 1986, 163, 922–937.CrossRefPubMedGoogle Scholar
  18. 18.
    Kubo, T., Towele, C.A., Markin, H.J., Treadwell, B.V. Stress-induced proteins in chondrocytes from patients with osteoarthritis. Arthritis Rheum 1985, 28, 1140–1145.PubMedGoogle Scholar
  19. 19.
    Deguchi, Y., Negoro, S., Kishimoto, S. Heat shock protein synthesis in peripheral blood mononuclear cells from patients with systemic lupus erythematosus. Biochem Biophys Res Comm 1987, 148, 1063–1068.CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Y. Deguchi
    • 1
  • S. Kishimoto
    • 1
  1. 1.Third Department of Internal MedicineOsaka University School of MedicineOsakaJapan
  2. 2.Lab. Immunoregulation, National Institute of Allergy and Infectious DiseasesN.I.H.BethesdaUSA

Personalised recommendations