A 580 nm emission in haematoporphyrin-derivative solution and in treated cells
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Continuous wave and time-resolved fluorescence microscopy have been extensively used to characterize the behaviour of haematoporphyrin derivative (HPD) both in solution and in single cells. In this work, we report experimental evidence for the presence of a 580 nm-emitting species, occurring as a consequence of modifications in HPD induced by the cellular microenvironment. The fact that the formation of this ‘modified’ species seems to be favoured in tumour cells might increase sensitivity in the diagnostic use of HPD for the localization of early-stage tumours.
Key wordsHaematoporphyrin derivative 580 nm emission Time-resolved fluorescence microscopy
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