Journal of Plant Growth Regulation

, Volume 5, Issue 4, pp 183–189 | Cite as

Effects of the growth regulator 4PU-30 on growth, K+ content, and alkaloid production in tobacco callus cultures

  • Teresa Piñol
  • Javier Palazon
  • Teresa Altabella
  • Manuel Serrano
Article

Abstract

Growth, K+ content, and alkaloid production were compared in nonorganogenetic callus cultures ofNicotiana tabacum cv. Burley 21 grown at 25°C in the dark on two different media: a basal medium with 1 μM α-naphthaleneacetic acid and 1 μM kinetin, and one with 1 μM α-naphthaleneacetic acid and 1 μM 4PU-30 (N-(2-chloro-4-pyridyl)-N′-phenylurea). These callus tissues behaved differently not only in growth and K+ content but also in alkaloid production. In comparison to cultures grown with kinetin, those grown with 4PU-30 showed a significantly higher fresh weight and dry weight and K+ content during the growth period studied. The data clearly indicate a positive correlation between K+ uptake rate stimulated by 4PU-30 and cell enlargement rate. However, the alkaloid biosynthesis in the callus tissues was activated by the supply of kinetin and diminished by that of 4PU-30. It thus appears that cellular enlargement of meristematic tissue stimulated by 4PU-30 limited alkaloid production.

Keywords

Alkaloid Kinetin Callus Culture Enlargement Rate Callus Tissue 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Capelle SC, Mok DWS, Kirchner C, Mok MC (1983) Effects of thidiazoron on cytokinin autonomy and the metabolism of N6-(Δ2-isopentenil) [8-14C]adenosine in callus tissue ofPhaseolus lunatus L. Plant Physiol 73:796–802Google Scholar
  2. Cocucci MC, Dalla Rosa S (1980) Effects of canavanine on IAA- and fusicoccin-stimulated cell enlargment, proton extrusion and potassium uptake in maize coleoptiles. Physiol Plant 48:239–242Google Scholar
  3. Evans HJ, Sorger GJ (1966) Role of mineral elements with emphasis on the univalent cations. Annu Rev Plant Physiol 17:47–77Google Scholar
  4. Kinnersley AM, Dougall DK (1980) Correlation between the nicotine content of tobacco plants and callus cultures. Planta 149:205–206Google Scholar
  5. Letham DS (1971) Regulators of cell division in plant tissues. XII. A cytoquinin bioassay using excised radish cotyledons. Physiol Plant 25:391–396Google Scholar
  6. Mengel K, Kirby EA (1982) Principles of plant nutrition. International Potash Institute, Bern, SwitzerlandGoogle Scholar
  7. Mothes K, Engelbrecht L, Tschoepe KH, Hutschereuter-Treffitz G (1957) Root activity and nicotine formation. Flora 144:518–536Google Scholar
  8. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497Google Scholar
  9. Norris RF (1976) Expansion or radish cotyledons: An interaction between NaCl and cytokinins. Plant Sci Lett 6:63Google Scholar
  10. Ohta S, Matsui O, Yatazawa M (1978) Culture conditions for nicotine production in tobacco tissue culture. Agric Biol Chem 42:1245–1251Google Scholar
  11. Piñol MT, Palazón J, Serrano M (1984) Growth and nicotine content of tobacco callus cultures without organogenesis. Plant Sci Lett 36:219–223Google Scholar
  12. Piñol MT, Palazón J, Altabella T, Cusido R, Serrano M (1985) Effect of auxin on alkaloids, K+ and free amino acids content in cultured tobacco callus. Physiol Plant 65:299–304Google Scholar
  13. Schmid H, Serrano M (1948) Untersuchungen über die Nikotinbildung des Tabaks. Experientia 8:311–312Google Scholar
  14. Takahashi S, Shudo K, Okamoto T, Yamada K, Isogai Y (1978) Cytokinin activity of N-phenyl-N′-(4-pyridyl)urea derivates. Phytochemistry 17:1201–1207Google Scholar

Copyright information

© Springer-Verlag New York Inc 1987

Authors and Affiliations

  • Teresa Piñol
    • 1
  • Javier Palazon
    • 1
  • Teresa Altabella
    • 1
  • Manuel Serrano
    • 1
  1. 1.Departamento de Fisiología Vegetal, Facultad de FarmaciaCiudad UniversitariaBarcelonaSpain

Personalised recommendations