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Disk diffusion susceptibility tests with norfloxacin: confirmation of proposed interpretive criteria

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Abstract

One hundred and eighty three clinical isolates of aerobic bacteria were tested against norfloxacin by both agar dilution (WHO-ICS) and disk diffusion test procedures (standardized FDA single disk test). Two experimental 10μg norfloxacin disks were studied. Results were analyzed in terms of recently recommended breakpoints for clinical susceptibility (MIC ⩽ 16μg/ml, zone diameter ⩾ 17 mm) and resistance (MIC ⩾ 32μg/ml, zone diameter ⩽ 12 mm). Excellent correlation was demonstrated by statistical analysis between paired MIC and zone size values (average value for each MIC; r=−0.9782). An MIC of 16μg/ml was found to correlate with a zone of 10.4 mm. Application of the recommended zone size breakpoints resulted in prediction of 177 isolates as being susceptible while six (3.3 %) were predicted to be either intermediate or resistant. The findings of this study validate the earlier recommendations stated above.

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Authors and Affiliations

  1. Department of Pathology, Virginia Commonwealth University, 23298, Richmond, Virginia, USA

    S. J. Wood

  2. Department of Medicine, Medical College of virginia, Virginia Commonwealth University, 23298, Richmond, Virginia, USA

    S. Shadomy

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  1. S. J. Wood
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  2. S. Shadomy
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Wood, S.J., Shadomy, S. Disk diffusion susceptibility tests with norfloxacin: confirmation of proposed interpretive criteria. Eur. J. Clin. Microbiol. 2, 242–244 (1983). https://doi.org/10.1007/BF02029525

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