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Comparison of immunodiffusion and crossed-immunoelectrophoresis in the diagnosis of invasive candidiasis

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Abstract

A restrospective single blind study was conducted to compare the efficacy of crossed-immunoelectrophoresis using concanavalin A intermediate gel with double immunodiffusion tests in the diagnosis of invasive candidiasis. On the basis of cytoplasmic antibody detection, crossed-immunoelectrophoresis with concanavalin A differentiated reliably betweenCandida albicans fungemia and invasive candida infection, whereas double immunodiffusion did not. With regard to sensitivity, specificity, and predictive value crossed-immunoelectrophoresis with concanavalin A was superior to double immunodiffusion. Analysis of double immunodiffusion precipitin bands revealed fuzzy and sharp precipitin bands which corresponded to mannan (fuzzy) and cytoplasmic (sharp) antibodies in the crossed-immunoelectrophoresis with concanavalin A assay. Therefore, the finding of sharp bands in the double immunodiffusion procedure supports the diagnosis of invasive candidiasis.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, and Department of Infectious Diseases, Hahnemann University Medical School, 19102, Philadelphia, Pennsylvania, USA

    A. N. Vanmali, A. L. Lentnek, J. Franco & B. Z. Ngwenya

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  1. A. N. Vanmali
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  2. A. L. Lentnek
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  3. J. Franco
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  4. B. Z. Ngwenya
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Vanmali, A.N., Lentnek, A.L., Franco, J. et al. Comparison of immunodiffusion and crossed-immunoelectrophoresis in the diagnosis of invasive candidiasis. Eur. J. Clin. Microbiol. 2, 206–212 (1983). https://doi.org/10.1007/BF02029517

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