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Differentiation of the U-937 promonocytic cell line induced by phorbol myristate acetate or retinoic acid: Effect of aurothiomalate

  • Molecular Immunopathology
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Abstract

Tissue macrophages, which participate in chronic synovial inflammation, differentiate from haemopoietic precursors in bone marrow and subsequently in tissue. During this process, they acquire attributes which are essential for their function in inflammation. Modulation of this process may represent a means of regulating inflammatory competence of macrophages in inflammatory joint disease. The action of aurothiomalate (ATM), an anti-rheumatic gold compound, on the differentiation of a promonocytic cell line (U937) was, therefore, examined inin vitro systems. U937 cells exposed to retinoic acid (RA) for 4 days or to phorbol myristate acetate (PMA) for 2 days acquired characteristics of macrophages, including the capacity to produce superoxide (O −2 ), responsiveness to formyl-methionyl-leucyl-phenylalanine (fMLP) and reduced proliferation. The activity of transglutaminase also increased in RA-exposed cultures. The effect of ATM exposure on acquisition of these characteristics was small and differed between RA- and PMA-stimulated cells.

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Authors and Affiliations

  1. Department of Pharmacology, University of Western Australia, 6009, Nedlands, Western Australia, Australia

    David A. Joyce & James H. Steer

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  1. David A. Joyce
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  2. James H. Steer
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Joyce, D.A., Steer, J.H. Differentiation of the U-937 promonocytic cell line induced by phorbol myristate acetate or retinoic acid: Effect of aurothiomalate. Agents and Actions 37, 305–310 (1992). https://doi.org/10.1007/BF02028124

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  • DOI: https://doi.org/10.1007/BF02028124

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