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Suppressive effects of E3330, a novel quinone derivative, on tumor necrosis factor-α generation from monocytes and macrophages

  • Molecular Immunopathology
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Abstract

E3330 {(2E)-3-[5-(2,3-dimethoxy-6-methyl-1,4-benzoquinoyl)]-2-nonly-2-propenoic acid}, a novel synthesized hepatoprotective compound, has suppressive effects on tumor necrosis factor-α (TNF-α) generation from monocytes/macrophagesin vitro. E3330 (1–100 μM) reduced lipopolysaccharide (LPS, 10 mg/ml or 1μg/ml)-induced TNF-α generation from rat resident andPropionibacterium acnes (P. acnes)-elicited peritoneal macrophages, rat and human monocytes, rat Kupffer cells, and splenic mononuclear cells in a concentration-dependent manner. E3330 also (1–100 μM) suppressed TNF-α generation stimulated with egg-albumin immune complex in ratP. acnes-elicited peritoneal macrophages. Northern blot analysis showed that LPS-induced expression of TNF-α messenger RNA (mRNA) in human blood monocytes was suppressed by E3330. These findings indicate that E3330 has a suppressive effect on TNF-α generation from monocytes/macrophages, regardless of origin or species, and this effect is based in part on the suppression of TNF-α mRNA expression.

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Authors and Affiliations

  1. Tsukuba Research Laboratories, Eisai Co. Ltd., Toukoudai 5-1-3, 300-26, Tsukuba-shi, Ibaraki, Japan

    K. Miyamoto, J. Nagakawa, I. Hishinuma, K. Hirota, M. Yasuda, T. Yamanaka, K. Katayama & I. Yamatsu

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  1. K. Miyamoto
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  2. J. Nagakawa
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  3. I. Hishinuma
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  4. K. Hirota
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  5. M. Yasuda
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  6. T. Yamanaka
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  7. K. Katayama
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  8. I. Yamatsu
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Miyamoto, K., Nagakawa, J., Hishinuma, I. et al. Suppressive effects of E3330, a novel quinone derivative, on tumor necrosis factor-α generation from monocytes and macrophages. Agents and Actions 37, 297–304 (1992). https://doi.org/10.1007/BF02028123

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  • DOI: https://doi.org/10.1007/BF02028123

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