Acute hypersensitivity to aerosolized histamine induced by aerosolized ovalbumin in guinea pigs
- 23 Downloads
Acute airway hyperresponsiveness can be induced after exposure to aerosolized ovalbumin in sensitized guinea pigs. The purpose of the present studies was to determine if “pro-inflammatory agents” would potentiate and prolong antigen-induced pulmonary hyperresponsiveness to histamine in guinea pigs. Guinea pigs were sensitized to aerosolized ovalbumin by exposing them to a 3 min aerosol, generated ultrasonically from a 10% ovalbumin solution on day 0 and day 7. On day 13 the guinea pigs were exposed to a 3 min aerosol of deionized water or a pro-inflammatory agent (1 μg/ml PAF, 1 mg/ml LPS, or 4%B. pertussis vaccine). Twenty-four hours later, on day 14, the conscious guinea pigs were challenged with a 3 min aerosolized ovalbumin exposure (under isoproterenol cover) and the individual guinea pig responsiveness to aerosolized histamine was determined 2 and 24h later in an anesthetized modified Konzett-Rossler preparation. Under these experimental conditions, ovalbumin challenge to sensitized guinea pigs produced only an acute hyperresponsiveness (about a 3–10-fold shift) to aerosolized histamine, which lasted less than 24h. The pro-inflammatory agents neither potentiated nor prolonged the duration of the hyperresponsiveness.
KeywordsDeionized Water Histamine Pertussis Isoproterenol Airway Hyperresponsiveness
Unable to display preview. Download preview PDF.
- A. N. Payne and G. De Nucci,Anaphylaxis in guinea pigs induced by ovalbumin aerosol: in vivo and in vitro methods. J. Pharmacol. Meth.17, 83–90 (1987).Google Scholar
- H. Takizawa, A. Ishii, S. Suzuki, J. Shiga and T. Miyamoto,Bronchoconstriction induced by platelet-activating factor in the guinea pig and its inhibition by CV-3988, a PAF antagonist: serial changes in findings of lung histology and bronchoalveolar lavage cell population. Int. Arch. Allergy Appl. Immunol.86, 375–382 (1988).PubMedGoogle Scholar
- G. Folkerts and F. P. Nijkamp,Induction of guinea pig respiratory airway hyperreactivity by Hemophilus influenzae: role of the histaminergic and cholinergic receptor systems. Agents and Actions17, 399–400 (1985).Google Scholar