Agents and Actions

, Volume 37, Issue 3–4, pp 198–200 | Cite as

Acute hypersensitivity to aerosolized histamine induced by aerosolized ovalbumin in guinea pigs

  • T. H. Gieske
  • L. E. Baugh
Pulmonary Research Group Meeting On Animal Models of Airway Hyperreactivity and Late-Phase Responses
  • 23 Downloads

Abstract

Acute airway hyperresponsiveness can be induced after exposure to aerosolized ovalbumin in sensitized guinea pigs. The purpose of the present studies was to determine if “pro-inflammatory agents” would potentiate and prolong antigen-induced pulmonary hyperresponsiveness to histamine in guinea pigs. Guinea pigs were sensitized to aerosolized ovalbumin by exposing them to a 3 min aerosol, generated ultrasonically from a 10% ovalbumin solution on day 0 and day 7. On day 13 the guinea pigs were exposed to a 3 min aerosol of deionized water or a pro-inflammatory agent (1 μg/ml PAF, 1 mg/ml LPS, or 4%B. pertussis vaccine). Twenty-four hours later, on day 14, the conscious guinea pigs were challenged with a 3 min aerosolized ovalbumin exposure (under isoproterenol cover) and the individual guinea pig responsiveness to aerosolized histamine was determined 2 and 24h later in an anesthetized modified Konzett-Rossler preparation. Under these experimental conditions, ovalbumin challenge to sensitized guinea pigs produced only an acute hyperresponsiveness (about a 3–10-fold shift) to aerosolized histamine, which lasted less than 24h. The pro-inflammatory agents neither potentiated nor prolonged the duration of the hyperresponsiveness.

Keywords

Deionized Water Histamine Pertussis Isoproterenol Airway Hyperresponsiveness 

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Copyright information

© Birkhäuser Verlag 1992

Authors and Affiliations

  • T. H. Gieske
    • 1
  • L. E. Baugh
    • 1
  1. 1.Marion Merrell Dow Research InstituteCincinnatiUSA

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