Summary
Pregnant rats were exposed to absorbed doses of 0.5, 1, 2, and 4 Gy of60Co-γ rays either on day 8 or on day 12 p.c.. The embryos were collected on day 18 p.c.. Irradiations both on day 8 and 12 p.c. result in a dose dependent decrement of weight. The effect is larger for irradiation on day 8 p.c.. Caryometric studies of the neurons in the trigeminal ganglion show, on the other hand, that the reduction of nuclear sizes in this tissue is more substantial for an irradiation on day 12 p.c. when the ganglion is in a stage of formation. Cytophotometric determinations of the Feulgen-DNA content and determination of the RNA content by staining with chromic gallocyanin in combination with ribonuclease digestion lead to the conclusion that the irradiation induces no significant hyperploidy and that the irradiated neurons have the nuclear RNA content that is normal at this stage of gestation. This applies both to the irradiations on day 8 and on day 12 p.c.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Angulo, A. W.: A comparison of the growth and differentiation of the trigeminal ganglia with the cervical spinal ganglia in albino rat embryos. J. Comp. Neurol.95, 53–71 (1951)
Brizzee, K. R., Jacobs, L. A., Kharetchko, X., Sharp, J. C.: Quantitative histologic and behavioral studies on effects of foetal X-irradiation in developing cerebral cortex of white rat. In: Response of the nervous system to ionizing radiation. Haley, T. J., Snider, R. S. (eds.), pp. 27–41. New York, London: Academic Press 1962
Cowden, R. R., Curtis, S. K.: A comparison of four quantitative cytochemical methods directed toward demonstration of DNA. Histochemistry45, 299–308 (1975)
Deitch, A. D., Wagner, D., Richart, R. M.: Conditions influencing the intensity of the Feulgen reaction. J. Histochem. Cytochem.16, 371–379 (1968)
Dutt, M. K., Kaufmann, B. P.: Microspectrophotometry determination of amount of DNA in irradiated mammalian cells grow in vitro. Nucleus9, 21–33 (1966)
Hicks, S. P., Brown, B. L., D'Amato, C. J.: Regeneration and malformation in the nervous system, eye and mesenchyme of the mammalian embryo after radiation injury. Am. J. Pathol.33, 459–481 (1957)
Hicks, S. P.: Effects of ionizing radiation on the developing nervous system. In: Response of the nervous system to ionizing radiation. Haley, T. J., Snider, R. S. (eds.), pp. 157–162. New York, London: Academic Press 1962
Lawson, S. N., Caddy, K. W. T., Biscoe, T. J.: Development of rat dorsal root ganglion neurons. Studies of cell birthdays and changes in mean cell diameter. Cell. Tissue Res.153, 399–413 (1974)
Lison, L., Valeri, V.: L'erreur dans la détermination histophotométrique de l'acide desoxyribonucléique et la variation individuelle des noyaux en acide desoxyribonucléique. Chromosoma7, 497–507 (1956)
Martin, P. G., Murphree, R. L.: Growth in prenatally irradiated rats as indicated by changes in cell number and cellular materials. Radiat. Res.40, 330–340 (1969)
Martin, P. G.: The postnatal response of four organs to prenatal irradiation as measured by changes in nucleic acids and protein. Radiat. Res.48, 368–376 (1971a)
Martin, P. G.: Effect of exposure and exposure-rat (γ radiation) on the brain of the prenatal rat. Int. J. Radiat. Biol.20, 547–555 (1971b)
Mendelsohn, M. L.: The two-wavelength method for microspectrophotometry. II: A set of tables to facilitate the calculations. J. Biophys. Biochem. Cytol.4, 415 (1958)
Mularek, O.: Investigations on the nucleic acids content in the brain of neonatal rats x-ray irradiated at different periods of the foetal life. I.: Cytophotometric determination of the DNA content in neurocytes. Exp. Pathol. (Jena)2, 78–86 (1968a)
Mularek, O.: Investigations on the nucleic acids content in the brain of neonatal rats x-ray irradiated at different periods of the foetal life. II: The biochemical DNA and RNA assay in the nervous tissue. Exp. Pathol. (Jena)2, 128–133 (1968b)
Mularek, O.: Cytometrial investigations of neurocytes nuclei in the brains of neonatal rats exposed to x-ray irradiation during various periods of fetal life. Radiobiol. Radiother. (Berl.)9, 495–499 (1968c)
Noeske, K.: Zur Stoïchiometrie der Feulgen-Reaktion. Acta Histochem. (Jena) (Suppl.)9, 701–702 (1971)
Noeske, K.: Discrepancies between cytophotometry Feulgen values and deoxyribonucleic acid content. J. Histochem. Cytochem.19, 169–174 (1971)
Olkowski, K.: Quantitative studies of histones and DNA in the motoneurons of the spinal cord of mice after total-body gamma-irradiation. Strahlentherapie142, 365–370 (1971)
Pannese, E.: The histogenesis of the spinal ganglia. Adv. Anat. Embryol. Cell Biol.47, 1–97 (1974)
Patau, K.: Absorption microphotometry of irregular shaped objects. Chromosoma5, 341–362 (1952)
Rugh, R.: Major radiobiological concepts and effects of ionizing radiations on the embryo and fetus. In: Response of the nervous system to ionizing radiation. Haley, T. J., Snider, R. S. (eds.), pp. 3–26. New York, London: Academic Press 1962
Sandritter, W.: Methods and results in quantitative cytochemistry. In: Introduction to quantitative cytochemistry. Wied, G. (ed.), pp. 159–182. New York, London: Academic Press 1966
Sandritter, W., Kiefer, G., Rick, W.: Gallocyanin chromealum. In: Introduction to quantitative cytochemistry. Wied, G. (ed.), pp. 295–326. New York, London: Academic Press 1966
Savy, C.: Etude caryométrique et histochimique quantitative de l'ADN et de l'ARN nucléaires au niveau des neurones du ganglion de Gasser du foetus de rat après irradiation embryonnaire. Thèse de 3ème cycle, 1976
Savy, C., Tamboise, E.: Effets cytologiques de divers fixateurs sur les neurones du ganglion de Gasser du foetus de rat. J. Hirnforsch.19, 469–478 (1978)
Savy, C., Tamboise, E.: Aspects quantitatif et qualitatif de la réaction de Feulgen dans les neurones du ganglion de Gasser du foetus de rat. J. Hirnforsch.20, 311–318 (1979)
Tamboise, E., Tamboise, A.: Etude quantitative du développement d'embryons de rats soumis à l'irradiation in utero an 7ème jour 1/2 de la gestation. Bull. Assoc. Anat. (Nancy)49, 1673–1687 (1964)
Tamboise, E., Tamboise, A.: Les principaux effets des radiations ionisantes sur le déeveloppement et la croissance de l'embryon de mammifère. Bull. Inf. Scient. Techn.94, 59–89 (1965)
Tamboise, E., Tamboise, A.: Etude quantitative des modifications induites par les radiations ionisantes sur le ganglion de Gasser et ses cellules chez l'embryon de rat. Bull. Assoc. Anat. (Nancy)51, 979–988 (1966)
Tamboise, E., Tamboise, A.: Intérêt de l'étude de la teneur en acides nucléiques des tissus embryonnaires en fonction des perturbations de leur croissance provoquées par l'action des rayonnements γ du cobalt. 56ème Congrès International des Anatomistes. Nantes, 1971
Thomson, R. Y., Frazer, S. C.: The desoxyribonucleic acid content of individual rat cell nuclei. Exp. Cell Res.6, 367–383 (1954)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Savy, C., Tamboise, E. Effects ofγ-irradiation on embryonic development and nucleic acids in trigeminal neurons. Radiat Environ Biophys 17, 115–127 (1980). https://doi.org/10.1007/BF02027848
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02027848