Summary
Radiation action occurs over a broad timescale which extends from the very early physical processes associated with energy absorption to the very late biological effects, such as carcinogenesis which may not become apparent until many years later. The various temporal stages of radiation action are classified and their interrelationships described. Experimental projects in cellular radiation chemistry, including pulse radiolysis, are discussed, together with some applications of the techniques in this general area.
The paper also deals with some aspects of the oxygen effect in radiobiology and the mechanisms of its action. Various studies employing fast response techniques have been useful in verifying the role of fast free radical reactions in the oxygen effects and examples are given of some applications. Investigations with other hypoxic cell sensitizers, the electron affinic agents, are also briefly discussed, with an account of how studies of the timescale of radiation sensitization can be valuable in understanding mechanisms. Possible mechanisms of action of radiation sensitization by oxygen and other agents, including radical fixation and direct action processes, are considered in the light of evidence from some model systems.
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References
Powers, E. L., Tallentire, A.: The roles of water in the cellular effects of ionizing radiation. In: Actions chimiques et biologiques des radiations. Haissinsky, M. (ed.), pp. 1–67. Paris: Masson 1968
Dodd, N. J. F., Ebert, M.: Effects of ionizing radiation on dried spores of Osmunda Regalis I. Electron spin resonance study of spores. Int. J. Radiat. Biol.18, 451–461 (1970)
Alexander, P., Charlesby, A.: Physico-chemical methods of protection against ionizing radiations. In: Radiobiology symposium. Bacq, Z. M., Alexander, P. (eds.), p. 49–59. London: Butterworths 1954
Alper, T., Howard-Flanders, P.: The role of oxygen in modifying the radiosensitivity of E. coli B. Nature178, 987 (1956)
Shenoy, M. A., Asquith, J. C., Adams, G. E., Michael, B. D., Watts, M. E.: Time-resolved oxygen effects in irradiated bacteria and mammalian cells: a rapid-mix study. Radiat. Res.62, 498–512 (1975)
Adams, G. E., Michael, B. D., Asquith, J. C., Shenoy, M. A., Watts, M. E., Whillans, D. W.: Rapid-mixing studies on the time scale of radiation damage in cells. In: Symp. “Fast Response Techniques in Chemistry and Biology”, Proc. V. Int. Cong. Radiat. Res., Seattle, 1974. Radiation Research: Biomedical chemical and physical perspectives. Nygaard, O. F., Adler, H. I., Sinclair, W. K. (eds.), pp. 478–492. London: Academic Press 1975
Tallentire, A., Jones, A. B., Jacobs, G. B.: The radiosensitizing actions of ketonic agents and oxygen in bacterial spores suspended in aqueous and non-aqueous milieux. Isr. J. Chem.10, 1185–1197 (1972)
Millar, B. C., Fielden, E. M., Steele, J.: A biphasic radiation survival response of mammalian cells to molecular oxygen. Int. J. Radiat. Biol.36, 177–180 (1979)
Epp, E. R., Weiss, H., Ling, C. C.: Irradiation of cells by single and double pulses of high intensity irradiation: oxygen sensitization and diffusion kinetics. Curr. Top. Radiat. Res.11, 201–230 (1976)
Michael, B. D., Adams, G. E., Hewitt, H. B., Jones, W. B. G., Watts, M. E.: A post-effect of oxygen in irradiated bacteria: a sub-millisecond fast mixing study. Radiat. Res.54, 239–251 (1973)
Michael, B. D., Harrop, H. A., Maughan, R. L., Patel, K. B.: A fast kinetics study of the modes of action of some different radiosensitizers in bacteria. Br. J. Cancer37 (Suppl. III), 29–33 (1978)
Adams, G. E., Dewey, D. L.: Hydrated electrons and radiobiological sensitization. Biochem. Biophys. Res. Commun.12, 473–477 (1963)
Raleigh, J. A., Chapman, J. D., Borsa, J., Kremers, W., Reuvers, A. P.: Radiosensitization of mammalian cells by p-nitroacetophenone III. Effectiveness of nitrobenzene analogues. Int. J. Radiat. Biol.23, 377–387 (1972)
Simic, M., Powers, E. L.: Correlation of the efficiencies of some radiation sensitizers and their redox potentials. Int. J. Radiat. Biol.26, 87–90 (1974)
Meisel, D., Czapski, G.: One electron transfer equilibrium and redox potentials of radicals studied by pulse radiolysis. J. Phys. Chem.79, 1503–1509 (1975)
Meisel, D., Neta, P.: One-electron redox potentials of nitrocompounds and radiosensitizers. Correlation with spin densities of their radical anions. J. Am. Chem. Soc.97, 5198–5203 (1975)
Wardman, P., Clarke, E. D.: One-electron reduction potentials of substituted nitroimidazoles measured by pulse radiolysis. J. Chem. Soc. Faraday Trans. I.72, 1377–1390 (1976)
Adams, G. E., Flockhart, I. R., Smithen, C. E., Stratford, I. J., Wardman, P., Watts, M. E.: Electron-affinic sensitization. VII: A correlation between structures, one-electron reduction potentials and efficiencies of nitroimidazoles as hypoxic cell radiosensitizers. Radiat. Res.67, 9–20 (1976)
Adams, G. E., Clarke, E. D., Flockhart, I. R., Jacobs, R. S., Sehmi, D. S., Stratford, I. J., Wardman, P., Watts, M. E.: Structure-activity relationships in the development of hypoxic cell radiosensitizers: I. Sensitization efficiency. Int. J. Radiat. Biol.35, 133–150 (1979)
Adams, G. E., Wardman, P.: Free radicals in biology: the pulse radiolysis approach. In: Free radicals in biology, Vol. III. Pryor, W. A. (ed.), pp. 53–95. London: Academic Press 1977
Chapman, J. D., Webb, R. G., Borsa, J.: Radiosensitization of mammalian cells by paranitroacetophenone I. Characterization in asynchronous and synchronous population. Int. J. Radiat. Biol.19, 561–574 (1971)
Adams, G. E., Asquith, J. C., Dewey, D. L., Foster, J. L., Michael, B. D., Willson, R. L.: Electron-affinic sensitization II. Paranitroacetophenone: a radiosensitizer for anoxic bacterial and mammalian cells. Int. J. Radiat. Biol.19, 575–585 (1971)
Adams, G. E., Asquith, J. C., Watts, M. E., Smithen, C. E.: Radiosensitization of hypoxic cells in vitro: a water soluble derivative of paranitroacetophenone. Nature239, 23–24 (1972)
Whitmore, G. F., Gulyas, S., Varghese, A. J.: Studies on the radiation-sensitizing action of NDPP, A sensitizer of hypoxic cells. Radiat. Res.61, 325–341 (1975)
Whillans, D., Hunt, J.: Rapid-mixing studies of the mechanisms of chemical radiosensitization and protection in mammalian cells. Br. J. Cancer37 (Suppl. III), 38–41 (1978)
Scholes, G.: In: Molecular aspects of radiation biology. Proceedings 23rd Farkas Memorial Symposium. Stein, G. (ed.). Isr. J. Chem.10, 1107–1122 (1972)
Chapman, J. D., Dugle, D. L., Reuvers, A. P., Gillispie, C. J., Borsa, J.: Chemical radiosensitization studies with mammalian cells growing in vitro. In: Proc. V. Int. Cong. Radiat. Res., Seattle. Radiation Research: Biomedical, chemical and physical perspectives. Nygaard, O. F., Adler, H. I., Sinclair, W. K. (eds.), pp. 478–492. London: Academic Press 1975
Sapora, O., Fielden, E. M., Loverock, P. S.: A comparative study of the effect of two classes of radiosensitizer on the survival of several E. coli B and K12 mutants. Radiat. Res.69, 293–305 (1977)
Adams, G. E., Cooke, M. S.: Electron-affinic sensitization. I. A structural basis for chemical radiosensitizers in bacteria. Int. J. Radiat. Biol.15, 457–471 (1969)
Gräslund, A., Ehrenberg, A., Rupprecht, A.: Free radical formation inγ-irradiated oriented DNA containing electron-affinic radiosensitizers. Int. J. Radiat. Biol.31, 145–152 (1977)
Jameson, D. G., Adams, G. E.: Short-lived luminescence from solid DNA irradiated with a single 3-nanosecond electron pulse under oxic and anoxic conditions. (Submitted to Radiat. Res., 1979)
Fielden, E. M., Lillicrap, S. C.: In: Advances in Chemistry Series No. 81 - Radiation Chemistry I. Gould, R. F. (ed.), pp. 444–463. Washington: American Chemical Society 1968
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Adams, G.E., Jameson, D.G. Time effects in molecular radiation Biology. Radiat Environ Biophys 17, 95–113 (1980). https://doi.org/10.1007/BF02027847
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DOI: https://doi.org/10.1007/BF02027847