Skip to main content
SpringerLink
Log in

The use of angiostatic steroids to inhibit cartilage destruction in anin vivo model of granuloma-mediated cartilage degradation

  • Bone and Cartilage
  • Published:
Agents and Actions Aims and scope Submit manuscript

Abstract

Angiogenesis is an important component of the development of chronic inflammatory diseases such as rheumatoid arthritis. It is known that clinically used anti-rheumatic drugs exert, in part, effects on the angiogenic response. Little work, however, has investigated the potential of experimental angiostatic therapies in chronic inflammatory disease models. The effect of one such angiostatic treatment, cortisone combined with heparin, was tested in anin vivo model of granuloma-mediated cartilage degradation. Angiostatic treatment significantly retarded the growth of granulomatous tissue, mononuclear cell influx into the granuloma, and the degradation of juxtaposed cartilage. This correlated with a decrease in the vascularity of the granulomatous tissue. Modulation of this component of pathogenesis of “angiogenesis-dependent disease” may be useful as a new therapeutic approach.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. H. G. Fassbender and A. Simmling-Annefield,The potential aggressiveness of synovial tissue in rheumatoid arthritis. J. Pathol.139, 399–406 (1983).

    PubMed  Google Scholar 

  2. J. Folkman,How is blood vessel growth regulated in normal and neoplastic tissue? G. H. A. Clowes Memorial Award Lecture. Cancer Res.46, 467–473 (1986).

    PubMed  Google Scholar 

  3. P. R. Colville-Nash and D. L. Scott,Angiogenesis and rheumatoid arthritis: Pathogenic and therapeutic implications. Ann. Rheum. Dis.51 (7), 919–925 (1992).

    PubMed  Google Scholar 

  4. M. Takigawa, Y. Nishida, F. Suzuki, J. Kishi, K. Yamashita and T. Hayakawa,Induction of angiogenesis in chick yolksac membrane by polyamines and its inhibition by tissue inhibitors of metalloproteinases (TIMP and TIMP-2). Biochem. Biophys. Res. Commun.171, 1264–1271 (1990).

    PubMed  Google Scholar 

  5. D. Ingber, T. Fujita, S. Kishimoto, K. Sudo, T. Kanamaru, H. Brem and J. Folkman,Synthetic analogues of fumagillin that inhibit angiogenesis and suppress tumour growth. Nature348, 555–557 (1990).

    PubMed  Google Scholar 

  6. R. Crum, S. Szabo and J. Folkman,A new class of steroids inhibits angiogenesis in the presence of heparin or a heparin fragment. Science230, 1375–1378 (1985).

    PubMed  Google Scholar 

  7. J. Folkman, R. Langer, R. J. Linhardt, C. Haudenschild and S. Taylor,Angiogenesis inhibition and tumour regression caused by heparin or a heparin fragment in the presence of cortisone. Science221, 719–725 (1983).

    PubMed  Google Scholar 

  8. D. J. Peacock, M. L. Banquerigo and E. Brahn,Angiogenesis inhibition suppresses collagen arthritis. InProceedings of the 55th Annual Meeting of the American College of Rheumatology. Arth. Rheum,34 (9), Suppl, Abstract 74, S45, (1991).

  9. F. B. De Brito, A. R. Moore, M. J. G. Holmes and D. A. Willoughby,Cartilage damage by a granulomatous reaction in a murine species. Br. J. Exp. Path.68, 675–686 (1987).

    Google Scholar 

  10. F. B. De Brito, M. J. G. Holmes, S. L. Carney and D. A. Willoughby,Drug effects on a novel model of connective tissue breakdown. Agents and Actions21, 287–289 (1987).

    PubMed  Google Scholar 

  11. K. M. K. Bottomley, R. J. Griffiths, T. J. Rising and A. Steward,A modified rat air pouch model for evaluating the effects of compounds on granuloma-induced cartilage degradation. Br. J. Pharmacol.93, 627–635 (1988).

    PubMed  Google Scholar 

  12. J. F. Woessner,The determination of hydroxyproline in tissue and protein samples containing small proportions of imino acids, Arch. Biochem. Biophys.93, 440–447 (1961).

    PubMed  Google Scholar 

  13. P. J. Bailey, A. Sturm and B. Lopez-Ramos,A biochemical study of the cotton pellet granuloma in the rat: Effects of dexamethasone and indomethacin. Biochem. Pharmacol.31, 1213–1218 (1982).

    PubMed  Google Scholar 

  14. J. W. Woollen, R. Heyworth and P. G. Walker,Studies on glucosaminidase. Biochem. J.78, 111–116 (1961).

    PubMed  Google Scholar 

  15. M. Kimura, K. Amemiya, T. Yamada and J. Suzuki,Quantitative method for measuring adjuvant-induced granuloma angiogenesis in insulin treated diabetic mice. J. Pharmacobio. Dyn.9, 442–446 (1986).

    PubMed  Google Scholar 

  16. A. R. Moore, T. H. P. Hanahoe, F. M. Desa, D. W. Howat, C. L. Chander and D. A. Willoughby,A procedure for assaying the component parts of rat femoral head cartilage and it's application to an in vivo model of cartilage breakdown. Int. J. Immunopathol. Pharmacol.1, 5–10 (1988).

    Google Scholar 

  17. R. W. Farndale, C. A. Sayer and A. J. Barrett,A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage culture. Connect. Tissue Res.9, 247–251 (1982).

    PubMed  Google Scholar 

  18. F. P. Altman,Quantitative dehydrogenase histochemistry with special reference to the pentose shunt dehydrogenases. Prog. Histochem. Cytochem.4, 225 (1972).

    Google Scholar 

  19. B. Henderson,Quantitative cytochemical measurement of glyceraldehyde-3-phosphate dehydrogenase activity. Histochemistry48, 191–204 (1976).

    PubMed  Google Scholar 

  20. J. Chayen, L. Bitensky and R. G. Butcher,Lactate Dehydrogenase. InPractical Histochemistry, pp. 197–199. Wiley, London, 1973.

    Google Scholar 

  21. T. A. Springer,Adhesion receptors of the immune system. Nature346, 425–431 (1990).

    PubMed  Google Scholar 

  22. M. R. Windt and L. J. Rosenwasser,Human vascular endothelial cells produce interleukin-1. Lymphokine Res.3, 281a (1984).

    Google Scholar 

  23. F. R. Jirik, T. J. Podor, T. Hirano, T. Kishimoto, D. J. Loskutoff, D. A. Carson and M. Lotz,Bacterial lipopolysaccharide and inflammatory mediators augment IL-6 secretion by human endothelial cells. J. Immunol.1, 144–147 (1989).

    Google Scholar 

  24. A. Mantovani and E. Dejana,Cytokines as communication signals between leukocytes and endothelial cells. Immunol. Today10, 370–375 (1989).

    PubMed  Google Scholar 

  25. J. S. Pober and M. Gimbrone,Expression of Ia-like antigens by human vascular endothelial cells is inducible in vitro: Demonstration by monoclonal antibody binding and immunoprecipitation. Proc. Nat. Acad. Sci.79, 6641–6645 (1982).

    PubMed  Google Scholar 

  26. P. E. Dicorleto and D. F. Bowen-Pope,Cultured endothelial cells produce a platelet-derived growth factor-like protein. Proc. Natl. Acad. Sci.80, 1919–1923 (1983).

    PubMed  Google Scholar 

  27. R. G. Gryglewski, R. M. Botting and J. R. Vane,Mediators produced by the endothelial cell. Hypertension12, 530–548 (1988).

    PubMed  Google Scholar 

  28. E. A. Jaffe,Endothelial cells. InInflammation: Basic Principles and Clinical Correlates. (Eds. J. I. Gallin, I. M. Goldstein and R. Snyderman) pp. 559–576, Raven Press, New York 1988.

    Google Scholar 

  29. E-D. Jarasch, G. Bruder and H. W. Heid,Significance of xanthine oxidase in capillary endothelial cells. Acta Scand. Physiol.548 (suppl), 39–46 (1986).

    Google Scholar 

  30. D. A. Willoughby, M. P. S. Seed, A. R. Moore, P. R. Colville-Nash and C. L. Chander,New areas for therapeutic intervention in the treatment of rheumatic disease. Therapie46, 461–467 (1991).

    PubMed  Google Scholar 

  31. C. R. Stevens, D. R. Blake, P. Merry, P. A. Revell and J. R. Levick,A comparative study by morphometry of the microvasculature in normal and rheumatoid synovium. Arth. Rheum.34, 1508–1513 (1991).

    Google Scholar 

  32. M. Rooney, D. Condell, W. Quinlan, L. Daly, A. Whelan, C. Feighery and B. Bresnihan,Analysis of the histologic variation in rheumatoid arthritis. Arth. Rheum.31, 956–963 (1988).

    Google Scholar 

  33. J. Folkman and D. E. Ingber,Angiostatic steroids. Method of discovery and mechanism of action. Ann. Surg.206, 374–383 (1987).

    PubMed  Google Scholar 

  34. D. E. Ingber, J. A. Madri and J. Folkman,A possible mechanism for inhibition of angiogenesis by angiostatic steroids: Induction of capillary basement membrane dissolution. Endocrinology119(4), 1768–1775 (1986).

    PubMed  Google Scholar 

  35. N. G. Tanaka, N. Sakamoto, A. Tohgo, Y. Nishiyama and H. Ogawa,Inhibitory effects of anti-angiogenic agents on neovascularization and growth of the chorioallantoic membrane (CAM). The possibility of a new CAM assay for angiogenesis inhibition. Exp. Pathol.30, 143–150 (1986).

    PubMed  Google Scholar 

  36. D. W. Beck, J. J. Olson and R. J. Linhardt,Effect of heparin, heparin fragments, and corticosteroids on cerebral endothelial cell growth in vitro and in vivo. J. Neuropathol. Exp. Neurol.45, 503–512 (1986).

    PubMed  Google Scholar 

  37. R. Cariou, J. L. Harousseau and G. Tobelem,Inhibition of human endothelial cell proliferation by heparin and steroids. Cell. Biol. Int. Rep.12, 1037–1047 (1988).

    PubMed  Google Scholar 

  38. C. L. Stokes, P. B. Weisz, S. K. Williams and D. A. Lauffenburger,Inhibition of microvascular endothelial cell migration by beta-cyclodextrin tetradecasulfate and hydrocortisone. Microvasc. Res.40, 279–284 (1990).

    PubMed  Google Scholar 

  39. I. Harada, T. Kikuchi, Y. Shimomura, M. Yamamoto, H. Ohno and N. Sato,The mode of action of anti-angiogenic steroid and heparin. InAngiogenesis. (Eds. R. Steiner, P. B. Weisz and R. Langer), pp. 445–448, Birkhauser, Basel 1992.

    Google Scholar 

  40. C. L. Chander, P. R. Colville-nash, A. R. Moore, D. W. Howat, F. M. Desa and D. A. Wiloughby,The effects of heparin and cortisone on an experimental model of pannus. Int. J. Tissue React.11, 113–116 (1989).

    PubMed  Google Scholar 

  41. N. E. Robertson, C. M. Discafani, E. C. Downs, J. A. Hailey, O. Sarre, R. L. J. Runkle, T. L. Popper and M. L. Plunkett,A quantitative in vivo mouse model used to assay inhibitors of tumor-induced angiogenesis. Cancer Res.51, 1339–1344 (1991).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Experimental Pathology, The Medical College of St. Bartholomew's Hospital, Charterhouse Square, EC1M 6BQ, London, UK

    P. R. Colville-Nash, M. El-Ghazaly & D. A. Willoughby

Authors
  1. P. R. Colville-Nash
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. El-Ghazaly
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. A. Willoughby
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Colville-Nash, P.R., El-Ghazaly, M. & Willoughby, D.A. The use of angiostatic steroids to inhibit cartilage destruction in anin vivo model of granuloma-mediated cartilage degradation. Agents and Actions 38 (Suppl 1), 126–134 (1993). https://doi.org/10.1007/BF02027224

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02027224

Keywords

Search

Navigation