Journal of Plant Growth Regulation

, Volume 5, Issue 2, pp 101–109 | Cite as

Gibberellins in apical shoot meristems of flowering and vegetative sugarcane

  • P. H. Moore
  • R. P. Pharis
  • M. Koshioka
Article

Abstract

Gibberellins A1, A3, iso-A3, A4, A19, A20, and A36 were identified by gas chromatography-selected ion monitoring in apices of sugarcane (Saccharum spp. hybrids). Flowering apices (i.e., 2–4 cm panicle) contained 8–9 times more (estimated by bioassay) endogenous gibberellins A and iso-GA3 (ratio of 1:6:8, respectively; in total 51 ng g−1 fresh weight) than vegetative apices (6.4 ng g−1 fresh weight). Vegetative apices contained small but significant levels of GA19, which could not be detected in flowering apices; vegetative apices also contained approximately four times more of a GA36-like substance than flowering apices. Since the two apex types developed under the same photoperiod, the increased levels of GA and iso-GA3 and the reduced levels of GA19 and GA36-like substances are correlated with the flowering state rather than with photoperiod or photoperiod changes per se. Since there were relatively high levels of C19 GAs along with low levels of C20 GAs in flowering apices, and since the converse is true in vegetative apices, metabolism of C20 to C19 GAs may be enhanced in flowering apices.

Keywords

Flowering State Gibberellin Saccharum Apical Shoot Meristem Shoot Meristem 

Abbreviations

GA

gibberellin(s)

MS

mass spectrometry

fw

fresh weight

GC

gas liquid chromatography

GC/MS

combined gas chromatography-mass spectrometry

MeOH

methanol

MeTMSi

methyl ester-trimethylsilyl ether

PVP

polyvinylpyrrolidone

SIM

selected ion monitoring

Rt

retention time

SiO2

silica gel

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Copyright information

© Springer-Verlag New York Inc 1986

Authors and Affiliations

  • P. H. Moore
    • 1
  • R. P. Pharis
    • 2
  • M. Koshioka
    • 2
  1. 1.UDSA/ARS, Experiment StationHawaiian Sugar Planters' AssociationAieaUSA
  2. 2.Plant Physiology Research Group, Department of BiologyUniversity of CalgaryCalgaryCanada

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