Abstract
Gibberellins A1, A3, iso-A3, A4, A19, A20, and A36 were identified by gas chromatography-selected ion monitoring in apices of sugarcane (Saccharum spp. hybrids). Flowering apices (i.e., 2–4 cm panicle) contained 8–9 times more (estimated by bioassay) endogenous gibberellins A⅓ and iso-GA3 (ratio of 1:6:8, respectively; in total 51 ng g−1 fresh weight) than vegetative apices (6.4 ng g−1 fresh weight). Vegetative apices contained small but significant levels of GA19, which could not be detected in flowering apices; vegetative apices also contained approximately four times more of a GA36-like substance than flowering apices. Since the two apex types developed under the same photoperiod, the increased levels of GA⅓ and iso-GA3 and the reduced levels of GA19 and GA36-like substances are correlated with the flowering state rather than with photoperiod or photoperiod changes per se. Since there were relatively high levels of C19 GAs along with low levels of C20 GAs in flowering apices, and since the converse is true in vegetative apices, metabolism of C20 to C19 GAs may be enhanced in flowering apices.
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Abbreviations
- GA:
-
gibberellin(s)
- MS:
-
mass spectrometry
- fw:
-
fresh weight
- GC:
-
gas liquid chromatography
- GC/MS:
-
combined gas chromatography-mass spectrometry
- MeOH:
-
methanol
- MeTMSi:
-
methyl ester-trimethylsilyl ether
- PVP:
-
polyvinylpyrrolidone
- SIM:
-
selected ion monitoring
- Rt:
-
retention time
- SiO2 :
-
silica gel
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Moore, P.H., Pharis, R.P. & Koshioka, M. Gibberellins in apical shoot meristems of flowering and vegetative sugarcane. J Plant Growth Regul 5, 101–109 (1986). https://doi.org/10.1007/BF02025961
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DOI: https://doi.org/10.1007/BF02025961