Abstract
The potential adverse phytotoxic effects of the herbicide safeners CGA-92194 {α-[1,3-dioxolan-2-yl-methoxy)imino]benzeneacetonitrile}, cyometrinil [α-(cyanomethoxy)imino-benzeneacetonitrile] and flurazole [phenylmethyl 2-chloro-4-(trifluoromethyl)-5-thiazole-carboxylate] on selected metabolic processes of enzymatically isolated leaf cells of soybean [Glycine max (L.) Merr.] were compared in time- and concentration-course studies. CO2 fixation, protein synthesis, RNA synthesis, DNA synthesis, and lipid synthesis were assayed by the incorporation of NaH14CO3, [14C]-leucine, [14C]-uracil, [3H]thymidine, and [14C]-acetate, respectively, into the isolated cells. CGA-92194 and cyometrinil behaved similarly, and at low concentrations (0.1, 1, and 10 μM) they stimulated rather than inhibited the five metabolic processes assayed, following incubation periods of up to 2 h. At the highest concentration of 100 μM, both safeners inhibited all metabolic processes of the soybean leaf cells but neither compound exhibited rapid and distinct inhibitions as might be expected in the case of inhibition of a primary target site by a potent inhibitor. At low concentrations and early incubation periods (30 and 60 min), flurazole effects on all metabolic processes were also stimulatory rather than inhibitory. However, the stimulation of CO2 fixation by 0.1 and 1.0 μM was highly significant. At 100 μM flurazole was extremely potent on all metabolic processes of soybean leaf cells examined. At the 2-h incubation period, flurazole also inhibited all metabolic processes at concentrations lower than 100 μM. The sensitivity of the five metabolic processes to flurazole decreased in the following order: photosynthesis = lipid synthesis >DNA synthesis>protein synthesis>RNA synthesis.
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Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase inBeta vulgaris L. Plant Physiol 24:1–15
Ashton FM, De Villiers OT, Glenn RK, Duke WB (1977) Localization of metabolic sites of action of herbicides. Pestic Biochem Physiol 7:122–141
Blair AM (1979) The interaction of protectants with EPTC on field bean, and triallate on wheat. Ann Appl Biol 92:105–111
Blair AM, Parker C, Kasasian L (1976) Herbicide protectants and antidotes—a review. Pestic Artic News Sum 22:65–74
Chang TS, Merkle MG (1972) Oximes as seed safeners for grain sorghum (Sorghum bicolor) herbicides. Weed Sci 30:70–73
Davidson WE, Gagnon SA, Chrustensen MD, Dorr JE (1978) A new herbicide safener which permits effective grass control in sorghum. Proc West Soc Weed Sci 31:123–129
Dill TR, Turner WE, Nyffeler A, Quadranti M (1982) CGA-92194—A new safener to protect sorghum for metolachlor injury. Abstr Weed Sci Soc Am 22:20
Eastin EF (1972) Evaluation of a sorghum seed treatment to prevent injury from acetanilide herbicides. Agron J 64:556–557
Ellis, FJ, Peek JW, Boehle J Jr., Muller G (1980) Effectiveness of a new safener for protecting sorghum (Sorghum bicolor) from metolachlor injury. Weed Sci 28:1–5
Ezra G, Krochmal E, Gressel J (1982) Competition between a thio-carbamate herbicide and herbicide protectants at the level of uptake into maize cells in culture. Pestic Biochem Physiol 18:107–112
Gressel J (1984) Plant tissue culture systems for screening of plant growth regulators: hormones, herbicides and natural toxins. Adv Cell Culture 3:93–181
Gressel J, Ezra G, Jain SM (1982) Genetic and chemical manipulation of crops to confer tolerance to chemicals. In: McLaren JD (ed) Chemical manipulation of crop growth and development. Butterworths, London, pp 79–91
Guneyli E (1971) Factors affecting the action of 1,8-naphthalic anhydride in corn treated withS-ethyl dipropylthiocarbamate (EPTC). Diss Abstr Int (B) 32:1957–1958
Hatzios KK (1983) Herbicide antidotes: development, chemistry and mode of action. Adv Agron 36:265–316
Hatzios KK (1984a) Interactions between selected herbicides and protectants on corn (Zea mays). Weed Sci 31:51–58
Hatzios KK (1984b) Potential safeners for protecting sorghum (Sorghum bicolor (L.) Moench) against chlorsulfuron, flurazifop-butyl and sethoxydim. Weed Res 24:249–254
Hatzios KK, Howe CM (1982) Influence of the herbicides hexazinone and chlorsulfuron on the metabolism of isolated soybean leaf cells. Pestic Biochem Physiol 17:207–214
Hatzios KK, Zama P (1986) Physiological interactions between the herbicide EPTC and selected analogues of the antidote naphthalic anhydride on two hybrids of maize. Pestic Sci 17:000–000 (in press)
Hoffman OL (1978) Herbicide antidotes: concept to crop. Chemtech 8:488–492
Ketchersid ML, Merkle MG (1983) Effects of protectants on sorghum seedling respiration. Proc South Weed Sci Soc 36:384
Mozer TJ, Tiemeier DC, Jaworski EG (1983) Purification and Characterization of corn glutathioneS-transferase. Biochemistry 22:1058–1072
Nyffeler A, Gerber HR, Hensley JR (1980) Laboratory studies on the behavior of the herbicide safener CGA-43089. Weed Sci 28:6–10
Pallos FM, Casida JE (1978) Chemistry and action of herbicide antidotes, Academic Press, New York
Parker C (1983) Herbicide antidotes—a review. Pestic Sci 14:40–48
Ries SK (1976) Subtoxic effects on plants. In: Audus LJ (ed) Herbicides: physiology, biochemistry, ecology, vol 2. Academic Press, London, pp 313–344
Sacher RM, Lee LF, Schafer DE, Howe RK (1983) Synthesis and application of novel thiazoles as herbicide antidotes. In: Miyamoto J, Kearney PC (eds) Pesticide chemistry: human welfare and the environment, vol 1. Pergamon Press, Oxford, pp 164–168
Schafer DE, Brinker RJ, Radke RO (1980) Introduction of a new safening agent for alachlor on grain sorghum. Proc North Cent Weed Control Conf 35:67–71
Servaites JC, Ogren WL (1980) Single cell photosynthesis. Methods Enzymol 69:642–649
Thiessen EP, Stephenson GR, Anderson GW (1980) Factors influencing 1,8-naphthalic anhydride activity as an antidote in barban in oats. Can J Plant Sci 60:1005–1013
Warmund MR, England DC, Kerr HD (1981) Anatomical site of action of MON-4606 in milo,Sorghum bicolor. Proc North Cent Weed Control Conf 36:123–124
Warmund MR, Kerr HD, Peters EJ (1985) Lipid metabolism in grain sorghum (Sorghum bicolor) treated with alachlor plus flurazole. Weed Sci 33:25–28
Zama P, Hatzios KK (1985) Interactive effects of metolachlor and the safener CGA-92194 on the metabolism of sorghum protoplasts. Proc South Weed Sci Soc 38:441
Zilberstein A, Arzee T, Gressel J (1973) Thymidine incorporation intoPharbitis DNA: II. Adenosine enhancement in vivo; total incorporation versus autoradiography. Cell Differ 2:213–220
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Contribution No. 534, Department of Plant Pathology, Physiology and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg VA 24061 USA.
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Zama, P., Hatzios, K.K. Comparative effects of CGA-92194, cyometrinil, and flurazole on selected metabolic processes of isolated soybean leaf cells. J Plant Growth Regul 5, 59–72 (1986). https://doi.org/10.1007/BF02025957
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DOI: https://doi.org/10.1007/BF02025957