Stability of phenylacetic acid in liquid media
- 73 Downloads
The stability of phenylacetic acid (PAA) in H2O and B5 and MS culture media was determined by HPLC. There was no loss of PAA when a nonsterile 10 mM stock solution was held at 5°C for 2 months. PAA was stable to autoclaving in full-strength MS and B5 media. After storage at 5°C or after agitation at 125 rpm at room temperature for 28 days, 100% of the PAA remained in B5 medium. Under comparable conditions, up to 15% of the PAA was lost in MS medium.
KeywordsAuxin Transport Phenylacetic Acid Auxin Activity Plant Suspension Culture Maximum Injection Volume
- Abe H, Uchiyama M, Sato R (1974) Isolation of phenylacetic acid and its p-hydroxy derivative as auxin-like substances fromUndaria pinnatifida. Agric Biol Chem 38:897–898Google Scholar
- Dodds JH, Roberts LW (1985) Experiments in plant tissue culture, 2d ed. Cambridge University Press, LondonGoogle Scholar
- Milborrow BV, Purse JG, Wightman F (1975) On the auxin activity of phenylacetic acid. Ann Bot 39:1143–1146Google Scholar
- Zimmerman PW, Wilcoxon F (1935) Several chemical growth substances which cause initiation of roots and other responses in plants. Contrib Boyce Thompson Inst 7:209–229Google Scholar