Stability of phenylacetic acid in liquid media

  • V. Leuba
  • D. LeTourneau
  • D. Oliver
Short Communication

Abstract

The stability of phenylacetic acid (PAA) in H2O and B5 and MS culture media was determined by HPLC. There was no loss of PAA when a nonsterile 10 mM stock solution was held at 5°C for 2 months. PAA was stable to autoclaving in full-strength MS and B5 media. After storage at 5°C or after agitation at 125 rpm at room temperature for 28 days, 100% of the PAA remained in B5 medium. Under comparable conditions, up to 15% of the PAA was lost in MS medium.

Keywords

Auxin Transport Phenylacetic Acid Auxin Activity Plant Suspension Culture Maximum Injection Volume 

References

  1. Abe H, Uchiyama M, Sato R (1974) Isolation of phenylacetic acid and its p-hydroxy derivative as auxin-like substances fromUndaria pinnatifida. Agric Biol Chem 38:897–898Google Scholar
  2. Dodds JH, Roberts LW (1985) Experiments in plant tissue culture, 2d ed. Cambridge University Press, LondonGoogle Scholar
  3. Dunlap JR, Kresovich S, McGee RE (1986) The effect of salt concentration on auxin stability in culture media. Plant Physiol 81:934–936PubMedCrossRefGoogle Scholar
  4. Fregeau JA, Wightman F (1983) Natural occurrence and biosynthesis of auxins in chloroplast and mitochondrial fractions from sunflower leaves. Plant Sci Lett 32:23–34CrossRefGoogle Scholar
  5. Johnson CF, Morris DA (1987) Regulation of auxin transport in pea (Pisum sativum L.) by phenylacetic acid: Effects on the components of transmembrane transport of indol-3yl-acetic acid. Planta 172:400–407CrossRefGoogle Scholar
  6. Milborrow BV, Purse JG, Wightman F (1975) On the auxin activity of phenylacetic acid. Ann Bot 39:1143–1146Google Scholar
  7. Wightman F, Lighty DL (1982) Identification of phenylacetic acid as a natural auxin in the shoots of higher plants. Physiol Plant 55:17–24CrossRefGoogle Scholar
  8. Zimmerman PW, Wilcoxon F (1935) Several chemical growth substances which cause initiation of roots and other responses in plants. Contrib Boyce Thompson Inst 7:209–229Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1989

Authors and Affiliations

  • V. Leuba
    • 1
  • D. LeTourneau
    • 1
  • D. Oliver
    • 1
  1. 1.Department of Bacteriology and BiochemistryUniversity of IdahoMoscowUSA

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