Stability of phenylacetic acid in liquid media
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The stability of phenylacetic acid (PAA) in H2O and B5 and MS culture media was determined by HPLC. There was no loss of PAA when a nonsterile 10 mM stock solution was held at 5°C for 2 months. PAA was stable to autoclaving in full-strength MS and B5 media. After storage at 5°C or after agitation at 125 rpm at room temperature for 28 days, 100% of the PAA remained in B5 medium. Under comparable conditions, up to 15% of the PAA was lost in MS medium.
KeywordsAuxin Transport Phenylacetic Acid Auxin Activity Plant Suspension Culture Maximum Injection Volume
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