Abstract
A number of endogenous substrates have been found in the 27,000g meristematic root membrane fraction of pea seedlings. The identities of such proteins remain unknown, but their phosphorylation patterns change during germination. The presence of different protein kinase activities, or different protein phosphatase activities besides the occurrence of metabolic variations in membranes, have been indicated as key factors that could act simultaneously to give variations in protein phosphorylation pattern during germination. The absence, previously reported, of such protein kinase activity in membranes extracted from differentiated root tissue has been related to a change in protein composition. This fact leads to the hypothesis that protein phosphorylation of such membranes could have a physiological role at least in meristematic tissues. However, no clear-cut indication of which physiological role such protein phosphorylations fulfill emerges from present data. Nevertheless, the coincidence of onset of DNA synthesis and variation in protein phosphorylation pattern of these membranes suggests that these two events could have some physiological dependence.
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Chiatante, D., Levi, M., Sgorbati, S. et al. Variations of phosphorylation of protein membrane components in pea root meristems during germination. J Plant Growth Regul 8, 107–119 (1989). https://doi.org/10.1007/BF02025278
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DOI: https://doi.org/10.1007/BF02025278