Ethylene binding changes in apple and morning glory during ripening and senescence

  • Sylvia M. Blankenship
  • Edward C. Sisler


Ethylene binding sites were measured during fruit ripening and morning glory flower senescence. Little change in ethylene binding was noted during these developmental stages, except a slight decline during the later stages of fruit ripening or flower senescence. The concentration of ethylene required to achieve 50% saturation of the binding sites was 0.14 μl/liter for both apple pulp and morning glory flowers. Ethylene binding sites were calculated to be 3.2×10−11 moles/kg and 3.8×10−9 moles/kg in apple and morning glory, respectively. It does not appear that changes seen in ethylene sensitivity during fruit ripening can be readily ascribed to changes in the number of ethylene binding sites in the tissue.


Apple Fruit Scatchard Plot Ethylene Sensitivity Flower Senescence Morning Glory 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Bengochea T, Dodds JH, Evans DE, Jerie PH, Niepel B, Shaari AR, Hall MA (1980a) Studies on ethylene binding by cell-free preparations from cotyledons ofPhaseolus vulgaris L. I. Separation and characterization. Planta 148:397–406CrossRefGoogle Scholar
  2. Bengochea T, Acaster MA, Dodds JH, Evans DE, Jerie PH, Hall MA (1980b) Studies on ethylene binding by cell-free preparations from cotyledons ofPhaseolus vulgaris L. II. Effects of structural analogues of ethylene and of inhibitors. Planta 148:407–411CrossRefGoogle Scholar
  3. Beyer EM Jr, Sundin O (1978)14C2H4 metabolism in morning glory flowers. Plant Physiol 61:896–899PubMedGoogle Scholar
  4. Blankenship SM, Unrath CR (1988) Internal ethylene levels and maturity of ‘Delicious’ and ‘Golden Delicious’ apples destined for prompt consumption. J Am Soc Hort Sci 113:88–91Google Scholar
  5. Burg SP, Burg EA (1965) Ethylene action and the ripening of fruits. Science 148:1190–1196PubMedCrossRefGoogle Scholar
  6. Goren R, Sisler EC (1984) Ethylene binding: some parameters in excised tobacco leaves. Tobacco Sci 28:115–119Google Scholar
  7. Goren R, Sisler EC (1986) Ethylene-binding characteristics inPhaseolus, Citrus andLigustrum plants. Plant Growth Regul 4:43–54CrossRefGoogle Scholar
  8. Harkett PJ, Hulme AC, Rhodes MJC, Wooltorton LSC (1971) The threshold value for physiological action of ethylene on apple fruits. J Food Technol 6:39–45Google Scholar
  9. Romani R (1984) Respiration, ethylene, senescence and homeostasis in an integrated view of postharvest life. Can J Bot 62:2950–2955CrossRefGoogle Scholar
  10. Saltveit ME, Jr (1982) Procedures for extracting and analyzing internal gas samples from plant tissues by gas chromotography. Hort Sci 17:878–881Google Scholar
  11. Sisler EC (1979) Measurement of ethylene binding in plant tissue. Plant Physiol 64:538–542PubMedCrossRefGoogle Scholar
  12. Sisler EC (1982) Ethylene binding in normal, rin and nor mutant tomatoes. J Plant Growth Regul 1:219–226Google Scholar
  13. Sisler EC, Reid MS, Yang SF (1986) Effect of antagonists of ethylene action on binding of ethylene in cut carnations. Plant Growth Regul 4:213–218CrossRefGoogle Scholar
  14. Sisler EC, Wood C (1987) Ethylene binding and evidence that bindingin vivo andin vitro is to the physiological receptor. In: Klambt D (ed) Plant hormone receptors 1987. NATO ASI Series, Vol. H10. Springer-Verlag, Berlin, Heidelberg, pp 239–248Google Scholar
  15. Smith AR, Robertson D, Sanders IO, Williams RAN, Hall MD (1987) Ethylene binding sites. In: Klambt D (ed) Plant hormone receptors. Springer-Verlag, Berlin, pp 229–238Google Scholar
  16. Venis MA (1985) Hormone binding sites in plants. Longman, New York pp 24–28Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1989

Authors and Affiliations

  • Sylvia M. Blankenship
    • 1
  • Edward C. Sisler
    • 1
  1. 1.Departments of Horticultural Science and BiochemistryNorth Carolina State UniversityRaleighUSA

Personalised recommendations