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Studies on cell motility in inflammation. I. The chemotactic activity of experimental, immunological and non-immunological, inflammatory exudates

  • Immunosuppression and Inflammation
  • Research Reports
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Abstract

The accumulation of leucocytes at the site of inflammation may be brought about by chemotaxis or proliferation in the extravascular tissues. The present paper focuses on the chemotactic properties of different types of experimental inflammatory pleural exudates, using a modified Boyden chamber. The time-course of carrageenan-induced exudate chemotactic activity for polymorphs was maximat at 4 h, thereafter diminishing towards 24 and 48 h. Chemotactic activity for mononuclear cells remained unchanged throughout the 4–48 h time-course. Heating the exudates to 56°C for 1 h partially reduced chemotactic activity. These results correlate well with the migration of polymorph and mononuclear cells into the pleural cavity during carrageenan-induced pleurisy. The potency of polymorph and mononuclear cell chemotactic activity of different exudates was of the following order: carrageenan > calcium pyrophosphate > reverse passive Arthus > dextran. These results are discussed in order to elucidate the differences between the underlying mechanisms responsible for leucocyte accumulation in different types of inflammatory reaction.

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References

  1. P.C. Wilkinson,Chemotaxis and Inflammation (Churchill-Livingston, Edinburgh and London, 1974).

    Google Scholar 

  2. E.L. Opie,Enzymes and Antienzymes of Inflammatory Exudates, J. Exp. Med.7, 316 (1905).

    Google Scholar 

  3. V. Menkin,Dynamica of Inflammation, (C. C. Thomas, Springfield, Ill., 1940).

    Google Scholar 

  4. R. Snyderman, J.K. Phillips andS.E. Mergenhagen,Biological Activity of Complement In Vivo. Role of C5 in the Accumulation of Polymorphonuclear Leucocytes in Inflammatory Exudates, J. Exp. Med.134, 1131 (1971).

    PubMed  Google Scholar 

  5. P.A. Ward andN.J. Zvaifzer,Complement-Derived Leukotactic Factors in Inflammatory Synovial Fluids of Humans, J. Clin Inv.50, 606 (1971).

    Google Scholar 

  6. F. Capasso, C.J. Dunn, S. Yamamoto, D.A. Willoughby andJ.P. Giroud,Further Studies on Carrageenan Induced Pleurisy in Rats, J. Path.116, 117 (1975).

    PubMed  Google Scholar 

  7. D.A. Willoughby, C.J. Dunn, S. Yamamoto, F. Capasso, D.A. Deporter andJ.P. Giroud,Calcium Pyrophosphate-Induced Pleurisy in Rats. A New Model of Acute Inflammation, Agents and Actions5, 35 (1975).

    PubMed  Google Scholar 

  8. S. Yamamoto, C.J. Dunn, D.A. Reporter, F. Capasso, D.A. Willoughby andE.C. Huskisson,A Model for the Quantitative Study of Immediate Hypersensitivity in Rats, Agents and Actions5, 375 (1975).

    Google Scholar 

  9. J.V. Hurley, G.B. Ryan andA. Friedman,The Mononuclear Response to Intrapleural Injection in the Rat, J. Path. Bact.91, 575 (1966).

    PubMed  Google Scholar 

  10. P.A. Ward, E.R. Unanue, S.J. Goralnick andG.F. Schreiner,Chemotaxis of Rat Lymphocytes. J. Immun.119, 416 (1977).

    PubMed  Google Scholar 

  11. R.A. Paz andW.G. Spector,The Mononuclear Cell Response to Injury, J. Path. Bact.84, 85 (1962).

    PubMed  Google Scholar 

  12. W.G. Spector, A.W. Lykke andD.A. Willoughby,A Quantitative Study of Leucocyte Emigration in Chronic Inflammatory Granulomata, J. Path. Bact.93, 101 (1967).

    PubMed  Google Scholar 

  13. K.M. Wynne, W.G. Spector andD.A. Willoughby,Macrophage Proliferation In Vitro Induced by Exudates, Nature253, 636 (1975).

    PubMed  Google Scholar 

  14. M. Adolphe, J. Fontagne, M. Pelletier andJ.P. Giroud,Production of DNA Synthesis in Rat Macrophages In Vitro by Inflammatory Exudate, Nature253, 637 (1975).

    Google Scholar 

  15. L.R. Tse andP. Phelps,Polymorphonuclear Leukocyte Motility In Vitro. V. Release of Chemotactic Activity Following Phagocytosis of Calcium Pyrophosphate Crystals, Diamond Dust and Urate Crystals, J. Lab. Clin. Med.76, 403 (1970).

    PubMed  Google Scholar 

  16. I. Spilberg, A. Gallacher andB. Mandell,Calcium Pyrophosphate Dihydrate (CPPD) Crystal-Induced Chemotactic Factor: Subcellular Localization, Role of Protein Synthesis and Phagocytosis, J. Lab. Clin. Med.89, 817 (1977).

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Experimental Pathology, St. Bartholomew's Hospital Medical College, London, England

    L. Parente, M. S. Koh, D. A. Willoughby & A. Kitchen

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  1. L. Parente
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  2. M. S. Koh
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  3. D. A. Willoughby
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  4. A. Kitchen
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Parente, L., Koh, M.S., Willoughby, D.A. et al. Studies on cell motility in inflammation. I. The chemotactic activity of experimental, immunological and non-immunological, inflammatory exudates. Agents and Actions 9, 190–195 (1979). https://doi.org/10.1007/BF02024733

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  • DOI: https://doi.org/10.1007/BF02024733

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