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Relationship between histamine-induced changes of cyclic AMP and mechanical activity on smooth muscle preparations of the guinea-pig ileum and the rabbit mesenteric artery

  • Histamine and Kinins
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Abstract

On guinea-pig ileum and rabbit mesenteric artery contracted by high potassium (100 mM) histamine produced relaxations which were inhibited by the H2-receptor antagonist metiamide. These results are thus indicative for the role of H2-receptors in mediating relaxation and for H1-receptors in mediating contraction on smooth muscle.

Time course studies for the relaxing and cyclic AMP responses to histamine showed that the cyclic AMP increase preceded the H2-receptor mediated relaxation. The cyclic AMP increase in response to histamine was prevented by metiamide, but remained unaffected by mepyramine on both the guinea-pig ileum and the rabbit mesenteric artery.

In addition, dose-response curves obtained on the mesenteric artery demonstrated that the H2-receptor mediated depressor responses coincided with cyclic AMP increases. Thus, these results gave clear-cut evidence that cyclic AMP is an intracellular metabolic event only implicit in the H2-receptor mediated relaxation, but not in the H1-receptor mediated contraction on smooth muscle preparations.

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Authors and Affiliations

  1. Childrens Hospital, University of Düsseldorf, Moorenstraße 5, D-4000, Düsseldorf, Federal Republic of Germany

    D. Reinhardt

  2. Institute of Pharmacology, University of Essen, Hufelandstr. 55, D-43, Essen, Federal Republic of Germany

    E. Ritter, R. Butzheinen & H. J. Schümann

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  1. D. Reinhardt
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  2. E. Ritter
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  3. R. Butzheinen
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  4. H. J. Schümann
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Reinhardt, D., Ritter, E., Butzheinen, R. et al. Relationship between histamine-induced changes of cyclic AMP and mechanical activity on smooth muscle preparations of the guinea-pig ileum and the rabbit mesenteric artery. Agents and Actions 9, 155–162 (1979). https://doi.org/10.1007/BF02024727

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  • DOI: https://doi.org/10.1007/BF02024727

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