Agents and Actions

, Volume 10, Issue 1–2, pp 124–131 | Cite as

Isolation and some properties of mast cells from the mesentery of the rat and guinea pig

  • Frederick L. Pearce
  • Madeleine Ennis
Histamine and Kinins Histamine Release

Abstract

A number of enzymes were screened for their ability to dissociate mesenteric tissues from the rat and guinea pig into their component cells. The bacterial enzyme collagenase was found to be the most satisfactory agent and a procedure based on the use of this protease was developed. The resulting suspensions contained 1–2% free mast cells and exhibited a low (ca. 5%) spontaneous release of histamine. The tissue cells contained less histamine than rat peritoneal mast cells and the guinea pig cells were smaller in size. Cells obtained from actively sensitized animals responded to antigenic challenge more strongly than the chopped tissue indicating that they were functionally intact. Rat mesenteric cells could be passively sensitized with homologous reaginic antibody and also responded to anti-rat IgE. The immunologically induced releases from rat mesenteric and peritoneal cells showed differing sensitivities to potentiation by phosphatidyl serine but the responses were directly comparable in the absence of this effect. Rat mesenteric cells also responded, but less effectively than the peritoneal cells, to the ionophore A23187, concanavalin A, ATP and basic secretagogues. They were, however, essentially refractory to the action of dextran. In contrast, guinea pig mast cells responded strongly only to the ionophore and weakly or not at all to the other agents. These results indicate marked inter-and intra-species differences in the reactivity of mast cells and suggest that rat peritoneal cells should not be used as the sole model for studying histamine secretion.

Keywords

Mast Cell Histamine Phosphatidyl Serine Peritoneal Cell Sole Model 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    J.L. Mongar andH.O. Schild,A Comparison of the Effects of Anaphylactic Shock and of Chemical Histamine Releasers, J. Physiol.118, 461–478 (1952).PubMedGoogle Scholar
  2. [2]
    B. Diamant,Observations on Anaphylactic and Compound 48/80-Induced Histamine Release from Guinea-Pig and Rat Lung Tissue in Vitro, Acta physiol. scand.55, 11–25 (1962).PubMedGoogle Scholar
  3. [3]
    J.R. Carstairs, M.M. Dale andJ.L. Mongar,The Isolation and Some Properties of Guinea Pig Mesenteric Mast Cells, Exp. Cell Res.81, 156–162 (1973).CrossRefPubMedGoogle Scholar
  4. [4]
    F.L. Pearce, H. Behrendt, U. Blum G. Poblete-Freundt, P. Pult, C. Stang-Voss andW. Schmutzler,Isolation and Study of Functional Mast Cells from Lung and Mesentery of the Guinea Pig, Agents and Actions7, 45–56 (1977).CrossRefPubMedGoogle Scholar
  5. [5]
    F.L. Pearce,The Use of Porous Plastic Filters in the Preparation of Cell Suspensions, Microscopica Acta79, 43–45 (1977).PubMedGoogle Scholar
  6. [6]
    I. Mota andI. Vugman,Effects of Anaphylactic Shock and Compound 48/80 on the Mast Cells of the Guinea Pig Lung, Nature177, 427–429 (1956).PubMedGoogle Scholar
  7. [7]
    G. Atkinson, M. Ennis andF.L. Pearce,The Effect of Alkaline Earth Cations on the Release of Histamine from Rat Peritoneal Mast Cells Treated with Compound 48/80 and Peptide 401 Brit. J. Pharmac.65, 395–402 (1979).Google Scholar
  8. [8]
    B.M. Ogilvie,Reagin-like Antibodies in Rats Infected with the Nematode Parasite Nippostrongylus brasiliensis, Immunology12, 113–131 (1966).Google Scholar
  9. [9]
    R. Keller,Concanavalin A, A Moel ‘Antigen’ for the in Vitro Detection of Cell-Bound Reaginic Antibody in the Rat, Clin. exp. Immunol.13, 139–147 (1973).PubMedGoogle Scholar
  10. [10]
    J.L. Mongar andH.O. Schild,Inhibition of the Anaphylactic Reaction, J. Physiol.135, 301–319 (1957).PubMedGoogle Scholar
  11. [11]
    P.A. Shore, A. Burkhalter andV.H. Cohn,A Method for the Fluorometric Assay of Histamine in Tissues, J. Pharmac. exp. Ther.127, 182–186 (1959).Google Scholar
  12. [12]
    L.J. Loeffler, W. Lovenberg andA Sjoerdsma,Effects of Dibutyryl-3′5′-cyclic Adenosine Monophosphate, Phosphodiesterase Inhibitors and Prostaglandin E 1 on Compound 48/80-Induced Histamine Release from Rat Peritoneal Mast Cells in Vitro, Biochem. Pharmac.20, 2287–2297 (1971).CrossRefGoogle Scholar
  13. [13]
    W. Kazimierczak andB. Diamant,Mechanisms of Histamine Release in Anaphylactic and Anaphylactoid Reactions, Prog. Allergy24, 295–365 (1978).PubMedGoogle Scholar
  14. [14]
    J.L. Mongar andP. Svec,The Effect of Phospholipids on Anaphylactic Histamine Release, Brit. J. Pharmac.46, 741–752 (1972).Google Scholar
  15. [15]
    A.D. Befus, F.L. Pearce, J. Gauldie, P. Horsewood, R.L. Goodacre, F. Cole, R.V. Heatley andJ. Bienenstock,Isolation and characteristics of Mast Cells from the Small Bowel Lamina Propria, in:The Mast Cell, Its Role in Health and Disease (Eds. J. Pepys and A.M. Edwards; Pitman Medical, Tunbridge Wells, 1979), 702–709.Google Scholar

Copyright information

© Birkhäuser Verlag 1980

Authors and Affiliations

  • Frederick L. Pearce
    • 1
  • Madeleine Ennis
    • 1
  1. 1.The Department of ChemistryUniversity College LondonLondonEngland

Personalised recommendations