Purification and immunochemical analysis of histidine decarboxylase from murine mastocytoma
Application of a new scheme of purification for histidine decarboxylase (E.C. 220.127.116.11) leads to a highly purified enzyme (5000 nmol/mg·h, i.p. 5.0, M.W.: 110 kD) with reasonable stability (rest activity 80% after 3 weeks at 6–8°C). A major protein contaminant seen on electrofocusing (i.p. 4.6) shows immunological identity with the enzyme-containing protein (i.p. 5.0) and might be involved in the aggregation of the enzyme.
KeywordsHistidine Major Protein Histidine Decarboxylase Immunochemical Analysis Reasonable Stability
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