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, Volume 10, Issue 6, pp 498–502 | Cite as

Selective antigen-stimulated release of proteolytic activity from rat mast cells

  • O. -H. Wilhelms
Histamine and Kinins Immunosuppression and Inflammation Cellular Secretion and Tissue Breakdown

Abstract

Stimulation of rat mast cell suspension from actively sensitized rats with antigen in vitro produced a parallel release of histamine and enzyme, probably proteolytic activity, which releasesp-nitrophenol from anl-tyrosine-p-nitrophenyl ester derivative (TPNE). The histamine and enzyme release correlated with respect to their dependence on antigen concentration, reaction time and inhibition by 2,4-DNP and papaverine. In contrast, more than 50% of total histamine but nearly no enzyme was released by the ionophore A 23.187 and C 48/80 (each ≤1 μg/ml). The enzyme was apparently secreted predominantly in a particular form. It was approximately 50% inactivated by heating for 1 h at 56°C or by incubation for 3 h at 37°C with the chymotrypsin inactivator tosyl-phenylalanine chloromethylketone (TPCK; 2.5×10−4M) or for 5 min at 37°C with benzyl sulphonyl fluoride (2.5×10−4M), which reacts with SH groups. Heating for 3 min at 100°C destroyed it completely. On the basis of these properties we suggest that the antigen-released enzyme is the known granulabound chymase from rat mast cells.

TPNE was not only a cleavable substrate for the enzymatic activity in the 800g cell supernatant following antigen stimulation, but also a strong inhibitor of the histamine release on administration before antigen (IC50∼10−6M). It appears that the same enzyme activity acts initially intracellularly as activator of the histamine secretion and then is subsequently released along with histamine as a further mediator. Extracellularly this enzyme may act as a modulator of inflammatory reactions in type I allergy both locally and systemically.

Keywords

Histamine Proteolytic Activity Histamine Release Chymotrypsin 800g Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser-Verlag 1980

Authors and Affiliations

  • O. -H. Wilhelms
    • 1
  1. 1.Medizinische ForschungBoehringer Mannheim GmbHMannheim 31FRG

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