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Neutrophil enzyme activities in carrageenan-induced inflammation in rats

  • Inflammation and Immunomodulation
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Abstract

During the course of carrageenan-induced inflammation in rats major changes were observed in the activities of neutrophil granule enzymes. The activities of three enzymes — gelatinase, collagenase and β-glucuronidase, the markers of three different types of granules, have been measured and compared to those of a control group of animals. Total collagenase and gelatinase activities of control rats were 59.4±3.6 (mean±SD) and 23.0±2.9 units/mg protein, respectively. Significantly reduced levels of both collagenase (35.6±2.5 units) (p<0.05) and gelatinase (7.1±0.7 units) (p<0.001) were measured in the blood neutrophils of inflamed rats; and the collagenase activity of neutrophils derived from inflamed pleural exudate was also significantly decreased to a levle of 19.7±1.8 units/mg protein (p<0.01). However, the gelatinase activity of exudate neutrophils did not differ from that of blood cells of inflamed rats.

In contrast, no change was found for the β-glucuronidase activity in blood neutrophils of control and inflamed rats.

These observations support the concept that during the inflammatory response in rats, neutrophils in the circulation may become activated as judged by the extracellular secretion of collagenase and gelatinase. Therefore, neutrophils accumulating in acute inflammatory lesions contain decreased levels of collagenolytic enzymes and the significance of this observation is discussed.

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Authors and Affiliations

  1. Department of Biochemistry, Institute of Rheumatology, 02-637, Warsaw, Poland

    I. Sopata, E. Wojtecka-Łukasik, J. Wize & S. Maśliński

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  1. I. Sopata
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  2. E. Wojtecka-Łukasik
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  3. J. Wize
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  4. S. Maśliński
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Sopata, I., Wojtecka-Łukasik, E., Wize, J. et al. Neutrophil enzyme activities in carrageenan-induced inflammation in rats. Agents and Actions 28, 89–92 (1989). https://doi.org/10.1007/BF02022986

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  • DOI: https://doi.org/10.1007/BF02022986

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