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Interrelationship between ethylene and growth regulators in the senescence of lettuce leaf discs

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Abstract

The interrelationship between ethylene and growth regulators in the senescence of romaine lettuce (Lactuca sativa L.) leaves was studied. Gibberellic acid (GA3), kinetin, and 3-indoleacetic acid (IAA) retarded chlorophyll loss from leaf discs which were floated on hormone solutions. Abscisic acid (ABA) and ethephon enhanced chlorophyll loss and antagonized the senescence-retarding effect of GA3 and kinetin. A high concentration of IAA (10−4 M) caused accelerated chlorophyll loss, whereas a similar concentration of kinetin neither retarded nor promoted chlorophyll loss. The ineffectiveness of IAA and kinetin at their supraoptimal concentrations in retarding leaf senescence was related to increased production of ethylene induced in the treated leaf discs. GA3 was the most effective in retarding chlorophyll loss and did not stimulate ethylene production at all. The senescence-enhancing effect of ABA was not mediated by ethylene. However, the moderately increased production of ethylene, induced by relatively high concentrations of ABA, could act synergistically with the latter to accelerate chlorophyll loss. It is proposed that the effectiveness of exogenously applied hormones, both in enhancing and retarding senescence, is greatly affected by the endogenous ethylene concentration of the treated plant tissue.

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References

  • Abeles FB (1972) Biosynthesis and mechanism of action of ethylene. Annu Rev Plant Physiol 23:259–292

    Article  CAS  Google Scholar 

  • Abeles FB, Rubinstein B (1964) Regulation of ethylene evolution and leaf abscission by auxin. Plant Physiol 39:963–969

    PubMed  CAS  Google Scholar 

  • Aharoni N (1978) Relationship between leaf water status and endogenous ethylene in detached leaves. Plant Physiol 61:658–662

    PubMed  CAS  Google Scholar 

  • Aharoni N, Anderson JD, Lieberman M (1979) Production and action of ethylene in senescing leaf discs: effect of indoleacetic acid, kinetin, silver ion, and carbon dioxide. Plant Physiol 64:805–809

    PubMed  CAS  Google Scholar 

  • Aharoni N, Back A, Ben-Yehoshua S, Richmond AE (1975) Exogenous gibberellic acid and the cytokinin isopentenyladenine retardants of senescence in romaine lettuce. J Am Soc Hortic Sci 100:4–6

    CAS  Google Scholar 

  • Aharoni N, Lieberman M (1979) Ethylene as a regulator of senescence in tobacco leaf discs. Plant Physiol 64:801–804

    PubMed  CAS  Google Scholar 

  • Aharoni N, Richmond AE (1978) Endogenous gibberellin and abscisic acid content as related to senescence of detached lettuce leaves. Plant Physiol 62:224–228

    PubMed  CAS  Google Scholar 

  • Aspinall D, Paleg LG, Addicott FT (1967) Abscision II and some hormone-regulated plant responses. Aust J Biol Sci 20:869–882

    CAS  Google Scholar 

  • Back A, Richmond AE (1971) Interrelations between gibberellic acid, cytokinins and abscisic acid in retarding leaf senescence. Physiol Plant 24:76–79

    Article  CAS  Google Scholar 

  • Beevers L (1968) Growth regulator control of senescence in leaf discs of nasturium (Tropaeolum majus). In: Wightman F, Setterfield G (eds) Biochemistry and Physiology of Plant Growth Substances. Runge Press, Ottawa, pp 1417–1435

    Google Scholar 

  • Burg SP (1962) The physiology of ethylene formation. Annu Rev Plant Physiol 13:265–302

    Article  CAS  Google Scholar 

  • Burg SP (1968) Ethylene, plant senescence and abscission. Plant Physiol 43:1503–1511

    PubMed  CAS  Google Scholar 

  • Burg SP, Burg EA (1968) Ethylene formation in pea seedlings: its relation to inhibition of bud growth caused by indole-3-acetic acid. Plant Physiol 43:1069–1074

    PubMed  CAS  Google Scholar 

  • Cathey HM (1968) Response of some ornamental plants to synthetic ABA. Proc Am Soc Hortic Sci 93:693–698

    Google Scholar 

  • Chin TY, Beevers L (1970) Changes in endogenous growth regulators in nasturtium leaves during senescence. Planta 92:178–188

    Article  CAS  Google Scholar 

  • Dennis DT, Stubbs M, Coultate TP (1967) The inhibition of brussel sprout leaf senescence by kinins. Can J Bot 45:1019–1024

    Article  CAS  Google Scholar 

  • Hall WC, Truchelut GB, Leinweber CL, Herrero FA (1957) Ethylene production by the cotton plant and its effect under experimental and field conditions. Physiol Plant 10:306–317

    Article  CAS  Google Scholar 

  • Kader AA (1985) Ethylene-induced senescence and physiological disorders in harvested horticultural crops. HortScience 20:54–56

    CAS  Google Scholar 

  • Lieberman M (1979) Biosynthesis and action of ethylene. Annu Rev Plan Physiol 30:533–591

    Article  CAS  Google Scholar 

  • Manos PJ, Goldthwaite J (1975) A kinetic analysis of the effects of gibberellic acid, zeatin, and abscisic acid on leaf tissue senescence inRumex. Plant Physiol 55:192–198

    PubMed  CAS  Google Scholar 

  • Mattoo AK, Aharoni N (1988) Ethylene and plant senescence. In: Noodén LD, Leopold AC (eds) Senescence and Aging in Plants. Academic Press, San Diego, pp 241–280

    Google Scholar 

  • McAfee JA, Morgan PW (1971) Rates of production and internal levels of ethylene in the vegetative cotton plant. Plant Cell Physiol 12:839–847

    CAS  Google Scholar 

  • Milborrow BV (1974) The chemistry and physiology of abscisic acid. Annu Rev Plant Physiol 25:259–307

    Article  CAS  Google Scholar 

  • Moran R, Porath D (1980) Chlorophyll determination in intact tissue using N,N-dimethylformamide. Plant Physiol 65:478–479

    Article  PubMed  CAS  Google Scholar 

  • Morgan PW, Hall WC (1964) Accelerated release of ethylene by cotton following application of indole-3-acetic acid. Nature 201:99

    Article  CAS  Google Scholar 

  • Noodén LD (1988) Abscisic acid, auxin and other regulators of senescence. In: Noodén LD, Leopold AC (eds) Senescence and Aging in Plants. Academic Press, San Diego, pp 329–355

    Google Scholar 

  • Noodén LD, Leopold AC (1978) Phytohormones and the endogenous regulation of senescence and abscission. In: Lathem DS, Goodwin PB, Higgins TJV (eds) Phytohormones and Related Compounds: A Comprehensive Treatise. Vol. 2. Elsevier/North-Holland, Amsterdam, pp 329–369

    Google Scholar 

  • Osborne DJ (1968) Ethylene as a plant hormone. In: Plant Growth Regulators. Monograph No. 31. Soc Chem Ind, London, pp 236–249

    Google Scholar 

  • Van Staden J, Cook EL, Noodén LD (1988) Cytokinins and senescence. In: Noodén LD, Leopold AC (eds) Senescence and Aging in Plants. Academic Press, San Diego, pp 281–328

    Google Scholar 

  • Yang SF, Hoffman NE (1984) Ethylene biosynthesis and its regulation in higher plants. Annu Rev Plant Physiol 35:155–189A0443016 00008 CS-SPJRNPDF [HEADSUP]

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Fruit and Vegetable Storage, Agricultural Research Organization, The Volcani Center, 50250, Bet Dagan, Israel

    Nehemia Aharoni

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  1. Nehemia Aharoni
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Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, No. 2571-E, 1988 series.

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Aharoni, N. Interrelationship between ethylene and growth regulators in the senescence of lettuce leaf discs. J Plant Growth Regul 8, 309–317 (1989). https://doi.org/10.1007/BF02021824

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  • DOI: https://doi.org/10.1007/BF02021824

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