Relationship of indole-3-butyric acid and adventitious rooting in M.26 apple (Malus Pumila mill.) shoots cultured in vitro
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The role of indole-3-butyric acid (IBA) in adventitious root formation was studied by analyzing the uptake and subsequent metabolism of IBA in shoots of M.26 apple (Malus pumila Mill.) rootstock grown in vitro. Roots were induced by exposing shoots to 4 μM IBA and [3H]IBA for 5 days in the dark and then transferring them to plant growth regulator (PGR)-free medium in the light until roots formed. Approximately 50% of the total radioactivity applied was taken up from the agar medium by the shoots during the 5-day incubation period in IBA. Indole-3-butyric acid metabolism was studied by extraction and high-performance liquid chromatographic (HPLC) separation of [3H]IBA and metabolites from the basal sections of treated shoots. The major [3H]IBA metabolite co-eluted with authentic [14C]indole-3-acetic acid (IAA) suggesting that IBA was converted to IAA in the shoots. The proportion of newly synthesized IAA present as conjugates was higher at the end of the 5-day IBA treatment period than after 13 days in PGR-free medium. There appeared to be no conjugation of IBA at any time.
KeywordsAdventitious Root Root Induction Adventitious Root Formation Basal Section Rooted Shoot
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- Epstein E, Lavee S (1984) Conversion of indole-3-butyric acid to indole-3-acetic acid by cuttings of grapevine (Vitis vinifera) and olive (Olea europaea). Plant Cell Physiol 25:597–703Google Scholar
- Hutchinson JF, Zimmerman RH (1987) Tissue culture of temperate fruit and nut trees. Hortic Rev 9:273–350Google Scholar
- James DJ, Thurnbon IJ (1979) Rapid in vitro rooting of the apple rootstock M.9. J Hortic Sci 56:15–20Google Scholar
- Jarvis BC (1986) Endogenous control of adventitious rooting in nonwoody cuttings. In: Jackson MB (ed) New root formation in plants and cuttings. Martinus Nijhoff, Dordrecht, Boston, Lancaster, pp 191–222Google Scholar
- Jones OP, Hopgood ME, O'Farrel D (1977) Propagation in vitro of M.26 apple rootstocks. J Hortic Sci 52:235–238Google Scholar
- Linsmaier E, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 50:107–112Google Scholar
- Quorin M, Lepoivre P, Boxus P (1977) Un premier bilan de 10 annees de recherches sur les cultures de meristemes et la multiplication in vitro de fruitiers ligneux. Compte rendu dus recherches 1976–1977 et rapports de synthese de la Station de Culture Fruiteres et Maraicheres, pp. 93–117. Centre de Recherches Agronomiques de l'Etat, Gembloux, (Belgique)Google Scholar