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Effect of fat-free diet on enzyme activities of rat testes

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Zeitschrift für Ernährungswissenschaft Aims and scope Submit manuscript

Zusammenfassung

  1. 1.

    Die bei Ultrazentrifugierung gewonnenen Membranfraktionen der Rattenhoden wurden auf ihre Succinatdehydrogenase-Aktivität (SDH) sowie für ihre Laktatdehydrogenaseisoenzym-Aktivität (LDHx) analysiert.

  2. 2.

    Die klinischen Symptome an EFA (essentielle Fettsäuren) der Ratten, die auf Futter von geringen EFA-Gehalt erzogen waren, manifestierten sich als Hypo- oder Aspermie. Die Anzeichen waren mit signifikanten Erhöhungen der C16, C18, C18∶1. Fettsäuren und mit einer Ermäßigung der C18∶2 2 and C18∶3 Fettsäuren verbunden.

  3. 3.

    Die Ermäßigungen der SDH und LDHx die durch Mangel an EFA verursacht wurden, waren von hoher statistischer Signifikanz. Die totale LDH-Aktivität (alle 6 LDH-Isoenzyme der Hoden erfassend) zeigte sich dagegen signifikant erhöht.

  4. 4.

    Die hier beschriebenen enzymatischen Veränderungen stimmen mit der Theorie überein, daß die EFA der Membranfraktionen eine Rolle in den biologischen Funktionen dieser Fraktionen spielen.

  5. 5.

    Es wird postuliert, daß die Resultate der Bestimmungen von SDH und LDHx der Hoden als ein Indikator von EFA-Mangel verwendet werden können.

Summary

  1. 1.

    Particulate fractions of rat testes obtained by ultracentrifugation were studied for their succinic acid dehydrogenase (SDH) and lactate dehydrogenase (LDH) (isoenzymic) activities. The maximal succinic acid dehydrogenase activity was found localized to mitochondrial subfraction D by ultracentrifugation, but the LDHx isoenzyme was preferentially localized to testicular mitochondrial subfraction E. These findings support the view of a particulate binding or localization of the above-mentioned two enzymes in testes.

  2. 2.

    In rats fed a diet deficient in essential fatty acids (EFA) the clinical symptoms of this deficiency, manifest as hypospermia or aspermia, were associated with a statistically significant increase in C16, C18 and C18∶1 fatty acids and a decrease in C18∶2 and C18∶3 fatty acids.

  3. 3.

    A statistically significant decrease in SDH and LDHx activities was shown to be induced by the deficiency. However, the total LDH activity (including that of all six isoenzymes of LDH in testes) was significantly increased.

  4. 4.

    The enzymio changes described are consistent with the idea that EFA, present in particulate fractions, may play a part in the biological (catalytic) function of these fractions.

  5. 5.

    It is suggested that estimation of SDH and LDHx activities of testes may be used as an index of the degree of deficiency in EFA.

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Author notes

  1. J. Clausen

    Present address: Neurochemical Institute Copenhagen N, Denmark

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    1. J. Clausen
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    Clausen, J. Effect of fat-free diet on enzyme activities of rat testes. Z Ernährungswiss 9, 322–332 (1969). https://doi.org/10.1007/BF02021513

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    • DOI: https://doi.org/10.1007/BF02021513

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